Ellagic acid could prevent experimentally induced oxidative stress in rats

Abstract

Aim: Reactive oxygen species (ROS) are produced during the metabolism of diethylnitrosamine (DEN) and phenobarbital (PB). Ellagic acid (EA) is a natural biologically active polyphenol with antioxidant activity. The present study was conducted to assess the preventive and protective capability of EA on DEN-PB-induced oxidative damage in rats. Materials and Methods: 60 Sprague-Dawley rats were assigned into 6 groups: Negative control, DEN, EA, DEN-PB (positive control), DEN-PB-EA (treatment), and EA-DEN-PB (pretreatment). 150 mg/kg DEN were administered to DEN, positive control, treatment and pretreatment groups. After two weeks, 500 ppm PB applied to positive control and treatment groups. 50 mg/kg/day EA were orally given to EA, and pretreatment groups for 8 weeks and treatment group was given for four weeks. After 8 weeks rats were sacrificed, liver and blood were subjected to biochemical evaluation. Results: DEN alone or with PB increased liver MDA levels significantly; however plasma MDA levels didn?t change. EA pretreatment decreased liver MDA levels (p Conclusion: It is considered that EA pretreatment could be more effective than EA treatment in DEN, PB induced oxidative stress in rats.

Keywords

• diethylnitrosamine
• ellagic acid
• oxidative stress
• phenobarbital