Optimization of κ-Selenocarrageenase Production by <i>Pseudoalteromonas sp</i>. Xi13 and Its Immobilization

Yashan Deng; Xixi Wang; Hui Xu; Cui Liu; Ran Li; Yuanyuan Zhang; Changfeng Qu; Jinlai Miao

doi:10.3390/molecules27227716

Molecules (Nov 2022)

Optimization of κ-Selenocarrageenase Production by <i>Pseudoalteromonas sp</i>. Xi13 and Its Immobilization

Yashan Deng,
Xixi Wang,
Hui Xu,
Cui Liu,
Ran Li,
Yuanyuan Zhang,
Changfeng Qu,
Jinlai Miao

Affiliations

Yashan Deng: Department of Pharmaceutical Engineering, College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao 266042, China
Xixi Wang: Key Laboratory of Marine Eco-Environmental Science and Technology, First Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, China
Hui Xu: Department of Pharmaceutical Engineering, College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao 266042, China
Cui Liu: Department of Pharmaceutical Engineering, College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao 266042, China
Ran Li: Department of Pharmaceutical Engineering, College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao 266042, China
Yuanyuan Zhang: Department of Pharmaceutical Engineering, College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao 266042, China
Changfeng Qu: Key Laboratory of Marine Eco-Environmental Science and Technology, First Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, China
Jinlai Miao: Key Laboratory of Marine Eco-Environmental Science and Technology, First Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, China

DOI: https://doi.org/10.3390/molecules27227716
Journal volume & issue: Vol. 27, no. 22
p. 7716

Abstract

Read online

The bioenzymatic production of selenium oligosaccharides addresses the problems resulting from high molecular weight and poor water solubility of κ-selenocarrageenan, and lays foundation for its application as adjuvant drugs for cancer treatment and food additive. κ-selenocarrageenase extracted from Pseudoalteromonas sp. Xi13 can degrade κ-selenocarrageenan to selenium oligosaccharides. The maximum optimized κ-selenocarrageenase activity using Response Surface Methodology (RSM) was increased by 1.4 times, reaching 8.416 U/mL. To expand applications of the κ-selenocarrageenase in industry, the preparation conditions of it in either lyophilized or immobilized form were investigated. The activity recovery rate of the lyophilized enzyme was >70%, while that of the immobilized enzyme was 62.83%. However, the immobilized κ-selenocarrageenase exhibits good stability after being reused four times, with 58.28% of residual activity. The selenium content of κ-selenocarrageenan oligosaccharides degraded by the immobilized κ-selenocarrageenase was 47.06 µg/g, 8.3% higher than that degraded by the lyophilized enzyme. The results indicate that the immobilized κ-selenocarrageenase is suitable for industrial applications and has commercial potential.

Published in Molecules

ISSN: 1420-3049 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Chemistry: Organic chemistry
Website: http://www.mdpi.com/journal/molecules

About the journal

Abstract

Keywords