Schisandrin B promotes hepatic differentiation from human umbilical cord mesenchymal stem cells

Meixian Jin; Xiao Yi; Xiaojuan Zhu; Wei Hu; Simin Wang; Qi Chen; Wanren Yang; Yang Li; Shao Li; Qing Peng; Mingxin Pan; Yi Gao; Shiyuan Xu; Ying Zhang; Shuqin Zhou

iScience (Feb 2024)

Schisandrin B promotes hepatic differentiation from human umbilical cord mesenchymal stem cells

Meixian Jin,
Xiao Yi,
Xiaojuan Zhu,
Wei Hu,
Simin Wang,
Qi Chen,
Wanren Yang,
Yang Li,
Shao Li,
Qing Peng,
Mingxin Pan,
Yi Gao,
Shiyuan Xu,
Ying Zhang,
Shuqin Zhou

Affiliations

Meixian Jin: Department of Anesthesiology, Zhujiang Hospital, Southern Medical University, Guangzhou 510000, China
Xiao Yi: Department of Gynecology, Zhujiang Hospital, Southern Medical University, Guangzhou 510000, China
Xiaojuan Zhu: Department of Anesthesiology, First People’s Hospital of Kashi, Kashi 844000, China
Wei Hu: Department of Anesthesiology, Zhujiang Hospital, Southern Medical University, Guangzhou 510000, China
Simin Wang: Department of Anesthesiology, Zhujiang Hospital, Southern Medical University, Guangzhou 510000, China
Qi Chen: Department of Anesthesiology, Zhujiang Hospital, Southern Medical University, Guangzhou 510000, China
Wanren Yang: General Surgery Center, Department of Hepatobiliary Surgery II, Guangdong Provincial Research Center for Artificial Organ and Tissue Engineering, Guangdong Provincial Key Laboratory of Viral Hepatitis Research, Institute of Regenerative Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou 510000, China
Yang Li: General Surgery Center, Department of Hepatobiliary Surgery II, Guangdong Provincial Research Center for Artificial Organ and Tissue Engineering, Guangdong Provincial Key Laboratory of Viral Hepatitis Research, Institute of Regenerative Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou 510000, China
Shao Li: General Surgery Center, Department of Hepatobiliary Surgery II, Guangdong Provincial Research Center for Artificial Organ and Tissue Engineering, Guangdong Provincial Key Laboratory of Viral Hepatitis Research, Institute of Regenerative Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou 510000, China
Qing Peng: General Surgery Center, Department of Hepatobiliary Surgery II, Guangdong Provincial Research Center for Artificial Organ and Tissue Engineering, Guangdong Provincial Key Laboratory of Viral Hepatitis Research, Institute of Regenerative Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou 510000, China
Mingxin Pan: General Surgery Center, Department of Hepatobiliary Surgery II, Guangdong Provincial Research Center for Artificial Organ and Tissue Engineering, Guangdong Provincial Key Laboratory of Viral Hepatitis Research, Institute of Regenerative Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou 510000, China
Yi Gao: General Surgery Center, Department of Hepatobiliary Surgery II, Guangdong Provincial Research Center for Artificial Organ and Tissue Engineering, Guangdong Provincial Key Laboratory of Viral Hepatitis Research, Institute of Regenerative Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou 510000, China
Shiyuan Xu: Department of Anesthesiology, Zhujiang Hospital, Southern Medical University, Guangzhou 510000, China; Corresponding author
Ying Zhang: General Surgery Center, Department of Hepatobiliary Surgery II, Guangdong Provincial Research Center for Artificial Organ and Tissue Engineering, Guangdong Provincial Key Laboratory of Viral Hepatitis Research, Institute of Regenerative Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou 510000, China; Corresponding author
Shuqin Zhou: Department of Anesthesiology, Zhujiang Hospital, Southern Medical University, Guangzhou 510000, China; Anesthesiology Department of The Second Affiliated Hospital, School of Medicine, The Chinese University of Hong Kong, Shenzhen & Longgang District People’s Hospital of Shenzhen, Shenzhen 518172, China; Corresponding author

Journal volume & issue: Vol. 27, no. 2
p. 108912

Abstract

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Summary: Human umbilical cord mesenchymal stem cells (UC-MSCs)-derived hepatocyte-like cells (HLCs) have shown great promise in the treatment of liver diseases. However, most current induction protocols yield hepatocyte-like cells with limited function as compared with primary hepatocytes. Schisandrin B (Sch B) is one of the main components of Schisandra chinensis, which can prevent fibrosis progression and promote liver cell regeneration. Herein, we investigated the effects of Sch B on hepatic differentiation of UC-MSCs. We found that treatment with 10 μM Sch B from the second stage of the differentiation process increased hepatic marker levels and hepatic function. Additionally, RNA-seq analysis revealed that Sch B promoted hepatic differentiation via activating the JAK2/STAT3 pathway. When transplanted HLCs into mice with CCL4-induced liver fibrosis, Sch B-treated HLCs exhibited significant therapeutic effects. This study provides an optimized hepatic differentiation protocol for UC-MSCs based on Sch B, yielding functioning cells for liver disease treatment.

Published in iScience

ISSN: 2589-0042 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science
Website: http://www.cell.com/iscience/home

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