Application of Novel VEGF-Loaded PCL-P Based Hybrid Copolymer Scaffold for Bone Regeneration

Abstract

Background: Polymeric materials are widely known as versatile materials, particularly for biomedical applications. The bioavailability, low toxicity, solubility, and permeability of this material play an essential role in biomedical implants, such as joint replacements, bone plates, ligaments, vascular grafts, intraocular lenses, heart valves, sutures, and dental implants. These materials can be formed as either biodegradable or other naturally bioactive polymers, which are composed of nano-sized particles in the composition of other materials. For example, PCL-P (HEMA-NIPAAm) is a thermosensitive, biodegradable, and biocompatible hydrogel for biomedical applications. In this study, we aimed to create a VEGF-loaded PCL-P (HEMA-NIPAAm) hybrid copolymer and analyze VEGF gene expression in human dental pulp stem cells (hDPSCs) that adhere to the copolymer. Methods: VEGF (0.1 Â g/mL) was loaded onto synthesized PCL-P (HEMA-NIPAAm), and hDPSCs were seeded into the scaffolds. To describe the hDPSC changes, VEGF mRNA, VEGF protein, and calcific deposition were examined. Results: VEGF-loaded PCL-P (HEMA-NIPAAm) increased VEGF mRNA and protein expression in hDPSCs compared to PCL-P (HEMA-NIPAAm) and cells with any scaffold (P>0.05). After 21 days of culture, the color intensity of alizarin red was significantly higher in VEGF-loaded PCL-P (HEMA-NIPAAm) cells than in those with any scaffold (P<0.05) and PCL-P (HEMA-NIPAAm) cells (P<0.05). Conclusion: Finally, thermosensitive and injectable PCL-P (HEMA-NIPAAM), including VEGF, enhanced the expression of genes associated with osteogenesis and angiogenesis in hDPSCs. Thus, these results imply that the thermo-sensitive PCL-P (HEMA-NIPAAm) scaffold may be a good option for future use as a VEGF carrier for hDPSC implantation.

Keywords

• vegf
• hydrogel
• scaffold
• gene
• human dental pulp stem cells