Protocol for 3D bioprinting of nanoparticle-laden hydrogels to enhance antibacterial and imaging properties

Mehdi Salar Amoli; Linqi Jin; Sarah Rezapourdamanab; Maher Saadeh; Yamini Singh; Liqun Ning; Boeun Hwang; Martin L. Tomov; Christopher N. LaRock; Morteza Mahmoudi; Holly Bauser-Heaton; Vahid Serpooshan

STAR Protocols (Dec 2024)

Protocol for 3D bioprinting of nanoparticle-laden hydrogels to enhance antibacterial and imaging properties

Mehdi Salar Amoli,
Linqi Jin,
Sarah Rezapourdamanab,
Maher Saadeh,
Yamini Singh,
Liqun Ning,
Boeun Hwang,
Martin L. Tomov,
Christopher N. LaRock,
Morteza Mahmoudi,
Holly Bauser-Heaton,
Vahid Serpooshan

Affiliations

Mehdi Salar Amoli: Department of Biomedical Engineering, Emory University School of Medicine and Georgia Institute of Technology, Atlanta, GA 30322, USA; Corresponding author
Linqi Jin: Department of Biomedical Engineering, Emory University School of Medicine and Georgia Institute of Technology, Atlanta, GA 30322, USA
Sarah Rezapourdamanab: Department of Biomedical Engineering, Emory University School of Medicine and Georgia Institute of Technology, Atlanta, GA 30322, USA
Maher Saadeh: Department of Biomedical Engineering, Emory University School of Medicine and Georgia Institute of Technology, Atlanta, GA 30322, USA
Yamini Singh: Department of Biomedical Engineering, Emory University School of Medicine and Georgia Institute of Technology, Atlanta, GA 30322, USA
Liqun Ning: Department of Biomedical Engineering, Emory University School of Medicine and Georgia Institute of Technology, Atlanta, GA 30322, USA; Department of Mechanical Engineering, Cleveland State University, Cleveland, OH 44115, USA
Boeun Hwang: Department of Biomedical Engineering, Emory University School of Medicine and Georgia Institute of Technology, Atlanta, GA 30322, USA
Martin L. Tomov: Department of Biomedical Engineering, Emory University School of Medicine and Georgia Institute of Technology, Atlanta, GA 30322, USA; Department of Pediatrics, Emory University School of Medicine, Atlanta, GA 30322, USA
Christopher N. LaRock: Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA, USA; Emory Antibiotic Resistance Center, Emory University School of Medicine, Atlanta, GA, USA; Division of Infectious Diseases, Department of Medicine, Emory University School of Medicine, Atlanta, GA, USA
Morteza Mahmoudi: Precision Health Program, Michigan State University, East Lansing, MI, USA
Holly Bauser-Heaton: Department of Biomedical Engineering, Emory University School of Medicine and Georgia Institute of Technology, Atlanta, GA 30322, USA; Department of Pediatrics, Emory University School of Medicine, Atlanta, GA 30322, USA; Children’s Healthcare of Atlanta, Atlanta, GA 30322, USA; Sibley Heart Center at Children’s Healthcare of Atlanta, Atlanta, GA, USA
Vahid Serpooshan: Department of Biomedical Engineering, Emory University School of Medicine and Georgia Institute of Technology, Atlanta, GA 30322, USA; Department of Pediatrics, Emory University School of Medicine, Atlanta, GA 30322, USA; Children’s Healthcare of Atlanta, Atlanta, GA 30322, USA; Corresponding author

Journal volume & issue: Vol. 5, no. 4
p. 103393

Abstract

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Summary: This protocol describes the preparation of a nanoparticle-encapsulated bioink capable of protecting tissue-engineered constructs against bacterial infections while also providing contrast for magnetic resonance (MR) imaging modalities. The report includes details of preparing the methacrylated gelatin-based bioinks and the incorporation of superparamagnetic iron oxide nanoparticles. A detailed protocol is presented for characterizing the bioink, evaluating cell response, and assessing its antibacterial effect. Overall, this article presents a robust approach for the development of antibacterial, MR-visible bioinks suitable for various tissue engineering applications.For complete details on the use and execution of this protocol, please refer to Theus et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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