Association of Gonadotropin-Releasing Hormone Receptor (GnRHR) gene (c.18085 T>G) and litter size in Ettawa crossbreed goat

Abstract

In the present study, the association of Gonadotropin-Releasing Hormone Receptor gene (GnRHR) and litter size were investigated using the PCR-RFLP method as a genetic marker candidate for litter size in 35 individuals from Ettawa crossbreed goats. This research has two steps conducted in the laboratory and in the field. Data on litter size and blood samples were collected from the field. In the laboratory was carried out DNA isolation, amplification, and restriction. Molecular analysis of DNA isolation were precise, the enzyme Msp1 (C*CGG) with a product length of 706 bp was used for amplification and restriction analysis, releaved TT genotype in GnRHR gene fragment. In the exon 3 of chromosome 6, the single nucleotide polymorphism (SNP) c.18085 T>G was found. Ettawa crossbreed goat in calculations using the hardy-weinberg law, insignificant results are obtained because the value is more than > 0.05 (db; 1) = 0.50. The genotypes and allele frequencies found in the Ettawa Crossbreed Goat GnRHR genes are monomorphic and the GnRHR gene did not associate with litter size. The conclusion is that GnRHR exon 3 gene (SNP) c.18085 T>G did not associate with litter size and can not be used as a genetic marker on Ettawa crossbred goats.

Keywords

• ettawa crossbreed goat
• litter size
• gnrhr gene
• msp1 enzyme
• pcr-rflp