Nature Communications (Jan 2022)
Expression of the transcription factor PU.1 induces the generation of microglia-like cells in human cortical organoids
- Bilal Cakir,
- Yoshiaki Tanaka,
- Ferdi Ridvan Kiral,
- Yangfei Xiang,
- Onur Dagliyan,
- Juan Wang,
- Maria Lee,
- Allison M. Greaney,
- Woo Sub Yang,
- Catherine duBoulay,
- Mehmet Hamdi Kural,
- Benjamin Patterson,
- Mei Zhong,
- Jonghun Kim,
- Yalai Bai,
- Wang Min,
- Laura E. Niklason,
- Prabir Patra,
- In-Hyun Park
Affiliations
- Bilal Cakir
- Department of Genetics, Yale Stem Cell Center, Yale School of Medicine
- Yoshiaki Tanaka
- Department of Genetics, Yale Stem Cell Center, Yale School of Medicine
- Ferdi Ridvan Kiral
- Department of Genetics, Yale Stem Cell Center, Yale School of Medicine
- Yangfei Xiang
- Department of Genetics, Yale Stem Cell Center, Yale School of Medicine
- Onur Dagliyan
- Department of Neurobiology, Harvard Medical School
- Juan Wang
- Department of Anesthesiology, Yale School of Medicine
- Maria Lee
- Department of Molecular, Cellular, Developmental Biology, Yale University
- Allison M. Greaney
- Department of Biomedical Engineering, Yale University
- Woo Sub Yang
- Department of Genetics, Yale Stem Cell Center, Yale School of Medicine
- Catherine duBoulay
- Department of Biology, Colby College
- Mehmet Hamdi Kural
- Department of Anesthesiology, Yale School of Medicine
- Benjamin Patterson
- Department of Genetics, Yale Stem Cell Center, Yale School of Medicine
- Mei Zhong
- Department of Cell Biology, Yale Stem Cell Center, Yale School of Medicine
- Jonghun Kim
- Department of Genetics, Yale Stem Cell Center, Yale School of Medicine
- Yalai Bai
- Department of Pathology, Yale School of Medicine
- Wang Min
- Interdepartmental Program in Vascular Biology and Therapeutics, Department of Pathology, Yale School of Medicine
- Laura E. Niklason
- Department of Anesthesiology, Yale School of Medicine
- Prabir Patra
- Department of Genetics, Yale Stem Cell Center, Yale School of Medicine
- In-Hyun Park
- Department of Genetics, Yale Stem Cell Center, Yale School of Medicine
- DOI
- https://doi.org/10.1038/s41467-022-28043-y
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 15
Abstract
The study of human microglia function in health and disease is limited by the availability of sound models. Here, the authors develop a method to generate functional microglia in human cortical organoids and investigate the role of human microglia during amyloid beta1-42- induced inflammation.
