PLoS ONE (Jan 2013)

High myeloperoxidase positive cell infiltration in colorectal cancer is an independent favorable prognostic factor.

  • Raoul A Droeser,
  • Christian Hirt,
  • Serenella Eppenberger-Castori,
  • Inti Zlobec,
  • Carsten T Viehl,
  • Daniel M Frey,
  • Christian A Nebiker,
  • Raffaele Rosso,
  • Markus Zuber,
  • Francesca Amicarella,
  • Giandomenica Iezzi,
  • Giuseppe Sconocchia,
  • Michael Heberer,
  • Alessandro Lugli,
  • Luigi Tornillo,
  • Daniel Oertli,
  • Luigi Terracciano,
  • Giulio C Spagnoli

DOI
https://doi.org/10.1371/journal.pone.0064814
Journal volume & issue
Vol. 8, no. 5
p. e64814

Abstract

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BACKGROUND: Colorectal cancer (CRC) infiltration by adaptive immune system cells correlates with favorable prognosis. The role of the innate immune system is still debated. Here we addressed the prognostic impact of CRC infiltration by neutrophil granulocytes (NG). METHODS: A TMA including healthy mucosa and clinically annotated CRC specimens (n = 1491) was stained with MPO and CD15 specific antibodies. MPO+ and CD15+ positive immune cells were counted by three independent observers. Phenotypic profiles of CRC infiltrating MPO+ and CD15+ cells were validated by flow cytometry on cell suspensions derived from enzymatically digested surgical specimens. Survival analysis was performed by splitting randomized data in training and validation subsets. RESULTS: MPO+ and CD15+ cell infiltration were significantly correlated (p<0.0001; r = 0.76). However, only high density of MPO+ cell infiltration was associated with significantly improved survival in training (P = 0.038) and validation (P = 0.002) sets. In multivariate analysis including T and N stage, vascular invasion, tumor border configuration and microsatellite instability status, MPO+ cell infiltration proved an independent prognostic marker overall (P = 0.004; HR = 0.65; CI:±0.15) and in both training (P = 0.048) and validation (P = 0.036) sets. Flow-cytometry analysis of CRC cell suspensions derived from clinical specimens showed that while MPO+ cells were largely CD15+/CD66b+, sizeable percentages of CD15+ and CD66b+ cells were MPO-. CONCLUSIONS: High density MPO+ cell infiltration is a novel independent favorable prognostic factor in CRC.