Antibacterial potential from periodontal dressing raw propolis Trigona itama bee based against Porphyromonas gingivalis: experimental research

Abstract

ABSTRACT Introduction: One of the procedures used in dentistry to address periodontal disease is periodontal surgery, which can lead to open wounds. Periodontal dressing is a dental material that can help with this issue by accelerate the healing of wounds. The aim of the in vitro study was to investigate the potential inhibition of periodontal dressing with the basic formulated from raw propolis Trigona itama bees against Porphyromonas gingivalis bacteria at various rates. Methods: This in vitro study was true experimental study with Porphyromonas gingivalis cultured in agar plate as sample which sample size was calculated using Federer formula. Periodontal dressing with raw propolis formulations were divided into eight groups of 85, 80, 75, 70, 65, 62.5, 60 and 57%, which were then compared with the 100% raw propolis, RESO-PAC™ group, COE-PAK™, and Baer formulations. Antibacterial testing was tested using zone of inhibition test and was calculated by adding up the zone of inhibition of Porphyromonas gingivalis. The data results were then analyzed using one-way ANOVA (p0.05. This means the data homogenous distributed. The results of statistical analysis using one-way ANOVA showed a p-value=0.001 (p0,05. Hal ini berarti data berdistribusi normal, Hasil uji homogenitas dengan levene’s statistic menunjukkan nilai signifikansi p>0,05, hal ini menunjukan bahwa data homogen. Hasil analisis statistik dengan menggunakan one way ANOVA menunjukkan p-value=0,001 (p<0,05) yang berarti terdapat perbedaan yang bermakna antar kelompok perlakuan. Hasil penelitian menunjukkan propolis mentah memiliki potensi antibakteri paling tinggi dengan diameter zona inhibisi 3.4667 mm setelah RESO-PAC™k, diikuti propolis mentah 85% dengan diameter 2.9167 mm dan propolis mentah 80% dengan diameter 2.5167 mm. Tidak ditemukan zona inhibisi bakteri pada konsentrasi 57% hingga 75%. Simpulan: Periodontal dressing menggunakan konsentrasi 85% memiliki aktivitas antibakteri tertinggi pada Porphyromonas gingivalis setelah propolis mentah 100% dan RESO-PAC™. Kata Kunci: Trigona itama, pengukuran antibakteri, periodontal dressing, propolis mentah

Keywords

• trigona itama, antibacterial assay, periodontal dressing, raw propolis, trigona itama, pengukuran antibakteri, periodontal dressing, propolis mentah