Extraction, purification and characterization of water soluble galactomannans from Mimosa pudica seeds

Abstract

Water soluble galactomannans from seed endosperm of Mimosa pudica L. was extracted and characterized (Fig. 1). Nuclear magnetic resonance spectroscopy and Gas Chromatography results revealed the presence of 4-linked mannose backbone with galactose side chains linked at the C6 position. Scanning Electron Micrographs showed smooth, elongated and irregular granular structure of galactomannan. Structural analysis by Attenuated total reflection infrared spectroscopy presented the Mannose to Galactose ratio while the X-ray diffraction studies showed the presences of A-type crystalline pattern of the galactomannan. Thermo Gravitimetric Analysis showed the three steps weight loss event and determined the thermal stability. The results showed that the extracted polysaccharides are typically amorphous, thermally stable and have desirable properties for industrial applications.