Frontiers in Microbiology (Sep 2020)
Temperate Bacteriophages (Prophages) in Pseudomonas aeruginosa Isolates Belonging to the International Cystic Fibrosis Clone (CC274)
- Antón Ambroa,
- Antón Ambroa,
- Lucia Blasco,
- Lucia Blasco,
- Carla López-Causapé,
- Rocio Trastoy,
- Rocio Trastoy,
- Laura Fernandez-García,
- Laura Fernandez-García,
- Ines Bleriot,
- Ines Bleriot,
- Manuel Ponce-Alonso,
- Manuel Ponce-Alonso,
- Manuel Ponce-Alonso,
- Olga Pacios,
- Maria López,
- Maria López,
- Maria López,
- Rafael Cantón,
- Rafael Cantón,
- Rafael Cantón,
- Timothy J. Kidd,
- German Bou,
- German Bou,
- German Bou,
- Antonio Oliver,
- Antonio Oliver,
- Antonio Oliver,
- Maria Tomás,
- Maria Tomás,
- Maria Tomás
Affiliations
- Antón Ambroa
- Microbiology Department-Research Institute Biomedical A Coruña (INIBIC), Hospital A Coruña (CHUAC), University of A Coruña (UDC), A Coruña, Spain
- Antón Ambroa
- Study Group on Mechanisms of Action and Resistance to Antimicrobials (GEMARA), Spanish Society of Infectious Diseases and Clinical Microbiology (SEIMC), Madrid, Spain
- Lucia Blasco
- Microbiology Department-Research Institute Biomedical A Coruña (INIBIC), Hospital A Coruña (CHUAC), University of A Coruña (UDC), A Coruña, Spain
- Lucia Blasco
- Study Group on Mechanisms of Action and Resistance to Antimicrobials (GEMARA), Spanish Society of Infectious Diseases and Clinical Microbiology (SEIMC), Madrid, Spain
- Carla López-Causapé
- Microbiology Department-Health Research Institute of the Baleairc Islands (IdISBa), Hospital Son Espases, Palma de Mallorca, Spain
- Rocio Trastoy
- Microbiology Department-Research Institute Biomedical A Coruña (INIBIC), Hospital A Coruña (CHUAC), University of A Coruña (UDC), A Coruña, Spain
- Rocio Trastoy
- Study Group on Mechanisms of Action and Resistance to Antimicrobials (GEMARA), Spanish Society of Infectious Diseases and Clinical Microbiology (SEIMC), Madrid, Spain
- Laura Fernandez-García
- Microbiology Department-Research Institute Biomedical A Coruña (INIBIC), Hospital A Coruña (CHUAC), University of A Coruña (UDC), A Coruña, Spain
- Laura Fernandez-García
- Study Group on Mechanisms of Action and Resistance to Antimicrobials (GEMARA), Spanish Society of Infectious Diseases and Clinical Microbiology (SEIMC), Madrid, Spain
- Ines Bleriot
- Microbiology Department-Research Institute Biomedical A Coruña (INIBIC), Hospital A Coruña (CHUAC), University of A Coruña (UDC), A Coruña, Spain
- Ines Bleriot
- Study Group on Mechanisms of Action and Resistance to Antimicrobials (GEMARA), Spanish Society of Infectious Diseases and Clinical Microbiology (SEIMC), Madrid, Spain
- Manuel Ponce-Alonso
- Study Group on Mechanisms of Action and Resistance to Antimicrobials (GEMARA), Spanish Society of Infectious Diseases and Clinical Microbiology (SEIMC), Madrid, Spain
- Manuel Ponce-Alonso
- Servicio de Microbiología, Hospital Ramón y Cajal, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, Spain
- Manuel Ponce-Alonso
- Spanish Network for Research in Infectious Diseases (REIPI), Seville, Spain
- Olga Pacios
- Microbiology Department-Research Institute Biomedical A Coruña (INIBIC), Hospital A Coruña (CHUAC), University of A Coruña (UDC), A Coruña, Spain
- Maria López
- Microbiology Department-Research Institute Biomedical A Coruña (INIBIC), Hospital A Coruña (CHUAC), University of A Coruña (UDC), A Coruña, Spain
- Maria López
- Study Group on Mechanisms of Action and Resistance to Antimicrobials (GEMARA), Spanish Society of Infectious Diseases and Clinical Microbiology (SEIMC), Madrid, Spain
- Maria López
- Spanish Network for Research in Infectious Diseases (REIPI), Seville, Spain
- Rafael Cantón
- Study Group on Mechanisms of Action and Resistance to Antimicrobials (GEMARA), Spanish Society of Infectious Diseases and Clinical Microbiology (SEIMC), Madrid, Spain
- Rafael Cantón
- Servicio de Microbiología, Hospital Ramón y Cajal, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, Spain
- Rafael Cantón
- Spanish Network for Research in Infectious Diseases (REIPI), Seville, Spain
- Timothy J. Kidd
- Child Health Research Centre, School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD, Australia
- German Bou
- Microbiology Department-Research Institute Biomedical A Coruña (INIBIC), Hospital A Coruña (CHUAC), University of A Coruña (UDC), A Coruña, Spain
- German Bou
- Study Group on Mechanisms of Action and Resistance to Antimicrobials (GEMARA), Spanish Society of Infectious Diseases and Clinical Microbiology (SEIMC), Madrid, Spain
- German Bou
- Spanish Network for Research in Infectious Diseases (REIPI), Seville, Spain
- Antonio Oliver
- Study Group on Mechanisms of Action and Resistance to Antimicrobials (GEMARA), Spanish Society of Infectious Diseases and Clinical Microbiology (SEIMC), Madrid, Spain
- Antonio Oliver
- Microbiology Department-Health Research Institute of the Baleairc Islands (IdISBa), Hospital Son Espases, Palma de Mallorca, Spain
- Antonio Oliver
- Spanish Network for Research in Infectious Diseases (REIPI), Seville, Spain
- Maria Tomás
- Microbiology Department-Research Institute Biomedical A Coruña (INIBIC), Hospital A Coruña (CHUAC), University of A Coruña (UDC), A Coruña, Spain
- Maria Tomás
- Study Group on Mechanisms of Action and Resistance to Antimicrobials (GEMARA), Spanish Society of Infectious Diseases and Clinical Microbiology (SEIMC), Madrid, Spain
- Maria Tomás
- Spanish Network for Research in Infectious Diseases (REIPI), Seville, Spain
- DOI
- https://doi.org/10.3389/fmicb.2020.556706
- Journal volume & issue
-
Vol. 11
Abstract
Bacteriophages are important in bacterial ecology and evolution. Pseudomonas aeruginosa is the most prevalent bacterial pathogen in chronic bronchopulmonary infection in cystic fibrosis (CF). In this study, we used bioinformatics, microbiological and microscopy techniques to analyze the bacteriophages present in 24 P. aeruginosa isolates belonging to the international CF clone (ST274-CC274). Interestingly, we detected the presence of five members of the Inoviridae family of prophages (Pf1, Pf4, Pf5, Pf6, Pf7), which have previously been observed in P. aeruginosa. In addition, we identified a new filamentous prophage, designated Pf8, in the P. aeruginosa AUS411.500 isolate belonging to the international CF clone. We detected only one prophage, never previously described, from the family Siphoviridiae (with 66 proteins and displaying homology with PHAGE_Pseudo_phi297_NC_016762). This prophage was isolated from the P. aeruginosa AUS531 isolate carrying a new gene which is implicated in the phage infection ability, named Bacteriophage Control Infection (bci). We characterized the role of the Bci protein in bacteriophage infection and in regulating the host Quorum Sensing (QS) system, motility and biofilm and pyocyanin production in the P. aeruginosa isogenic mutant AUS531Δbci isolate. The findings may be relevant for the identification of targets in the development of new strategies to control P. aeruginosa infections, particularly in CF patients.
Keywords
