Microorganisms (Jul 2022)

Substrate Utilisation and Energy Metabolism in Non-Growing <i>Campylobacter jejuni</i> M1cam

  • Emily Stoakes,
  • George M. Savva,
  • Ruby Coates,
  • Noemi Tejera,
  • Mark G. Poolman,
  • Andrew J. Grant,
  • John Wain,
  • Dipali Singh

DOI
https://doi.org/10.3390/microorganisms10071355
Journal volume & issue
Vol. 10, no. 7
p. 1355

Abstract

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Campylobacter jejuni, the major cause of bacterial foodborne illness, is also a fastidious organism that requires strict growth requirements in the laboratory. Our aim was to study substrate utilisation and energy metabolism in non-growing C. jejuni to investigate the ability of these bacteria to survive so effectively in the food chain. We integrated phenotypic microarrays and genome-scale metabolic modelling (GSM) to investigate the survival of C. jejuni on 95 substrates. We further investigated the underlying metabolic re-adjustment associated with varying energy demands on each substrate. We identified amino acids, organic acids and H2, as single substrates supporting survival without growth. We identified several different mechanisms, which were used alone or in combination, for ATP production: substrate-level phosphorylation via acetate kinase, the TCA cycle, and oxidative phosphorylation via the electron transport chain that utilised alternative electron donors and acceptors. The benefit of ATP production through each of these mechanisms was associated with the cost of enzyme investment, nutrient availability and/or O2 utilisation. C. jejuni can utilise a wide range of substrates as energy sources, including organic acids commonly used for marination or preservation of ingredients, which might contribute to the success of their survival in changing environments.

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