Effects of Exogenous Glycinebetaine on Growth and Ultrastructure of Salt-Stressed Rice Seedlings (Oryza sativa L.)

Abstract

The effects of exogenously applied glycinebetaine on the salt-stress-induced inhibition of growth and ultrastructural damages in rice seedlings were investigated. Glycinebetaine was not effective in alleviating the NaCl-induced inhibition of root growth and rather enhanced the NaCl-induced inhibition. However, it was found to alleviate the inhibition of shoot growth induced by NaCl stress. Concentrations of Na were higher in salt-stressed plants than in unstressed plants. Stressed plants receiving glycinebetaine had a significantly lower Na and higher K concentrations in the shoots than the plants grown without application of glycinebetaine. Salinity induced ultrastructural damages in leaf such as swelling of thylakoids, disintegration of grana stacking and intergranal lamellae and destruction of mitochondria (deficiency of cristae, swelling and vacuolation). Such damages were largely prevented by pretreatment with glycinebetaine resulting in greening of the plants. In roots, the epidermis, cortex and root cap were more sensitive to salt stress than the meristem and stele. The most frequently observed ultrastructural alteration due to NaCl salinity was the formation of many large vacuoles in the root tip and root cap cells. The number of mitochondria was increased and they were aggregated in the cytoplasm of the root tip and root cap cells by treatment with NaCl or NaCl plus glycinebetaine. Glycinebetaine could not prevent the NaCl-induced ultrastructural damages in root cells. The effects of glycinebetaine to mitigate the ultrastructural damages in the chloroplast and mitochondria induced by NaCl might be due to the production of many vacuoles in root cells which may act to store Na and decrease its accumulation in the shoot.

Keywords

• Glycinebetaine
• Growth
• Light microscopy
• NaCl
• Oryza sativa L
• Transmission electron microscopy (TEM)
• Ultrastructure