A nucleotide resolution map of Top2-linked DNA breaks in the yeast and human genome

William H. Gittens; Dominic J. Johnson; Rachal M. Allison; Tim J. Cooper; Holly Thomas; Matthew J. Neale

doi:10.1038/s41467-019-12802-5

Nature Communications (Oct 2019)

A nucleotide resolution map of Top2-linked DNA breaks in the yeast and human genome

William H. Gittens,
Dominic J. Johnson,
Rachal M. Allison,
Tim J. Cooper,
Holly Thomas,
Matthew J. Neale

Affiliations

William H. Gittens: Genome Damage and Stability Centre, University of Sussex
Dominic J. Johnson: Genome Damage and Stability Centre, University of Sussex
Rachal M. Allison: Genome Damage and Stability Centre, University of Sussex
Tim J. Cooper: Genome Damage and Stability Centre, University of Sussex
Holly Thomas: Genome Damage and Stability Centre, University of Sussex
Matthew J. Neale: Genome Damage and Stability Centre, University of Sussex

DOI: https://doi.org/10.1038/s41467-019-12802-5
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 16

Abstract

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Topoisomerase 2 (Top2) is known to resolve DNA topological stress through double strand breaks (DSBs), yet Top2 inhibition has been reported to result in a significant amount of single-strand breaks (SSBs). Here the authors develop CC-seq—a method that allows direct mapping of both Top2-linked SSBs and DSBs—and reveal a significant impact of primary DNA sequence on Top2 directed cleavage.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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