Polymers (Mar 2021)

Marine Algae Incorporated Polylactide Acid Patch: Novel Candidate for Targeting Osteosarcoma Cells without Impairing the Osteoblastic Proliferation

  • Salih Veziroglu,
  • Mustafa Ayna,
  • Theresa Kohlhaas,
  • Selin Sayin,
  • Jacek Fiutowski,
  • Yogendra Kumar Mishra,
  • Fatih Karayürek,
  • Hendrik Naujokat,
  • Eyüp Ilker Saygili,
  • Yahya Açil,
  • Jörg Wiltfang,
  • Franz Faupel,
  • Oral Cenk Aktas,
  • Aydin Gülses

DOI
https://doi.org/10.3390/polym13060847
Journal volume & issue
Vol. 13, no. 6
p. 847

Abstract

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Biodegradable collagen-based materials have been preferred as scaffolds and grafts for diverse clinical applications in density and orthopedy. Besides the advantages of using such bio-originated materials, the use of collagen matrices increases the risk of infection transmission through the cells or the tissues of the graft/scaffold. In addition, such collagen-based solutions are not counted as economically feasible approaches due to their high production cost. In recent years, incorporation of marine algae in synthetic polymers has been considered as an alternative method for preparation grafts/scaffolds since they represent abundant and cheap source of potential biopolymers. Current work aims to propose a novel composite patch prepared by blending Sargassum vulgare powders (SVP) to polylactide (PLA) as an alternative to the porcine-derived membranes. SVP-PLA composite patches were produced by using a modified solvent casting method. Following detailed material characterization to assess the cytocompatibility, human osteoblasts (HOBs) and osteosarcoma cells (SaOS-2) were seeded on neat PLA and SVP-PLA patches. MTT and BrdU assays indicated a greater cytocompatibility and higher proliferation for HOBs cultured on SVP-PLA composite than for those cultured on neat PLA. SaOS-2 cells cultured on SVP-PLA exhibited a significant decrease in cell proliferation. The composite patch described herein exhibits an antiproliferative effect against SaOS-2 cells without impairing HOBs’ adhesion and proliferation.

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