Generation of human induced pluripotent stem cell line (XWHNi004-A) from a male with APOE gene mutation

Pin Wang; Wenxian Sun; Jin Gong; Xiaodong Han; Chang Xu; Yufei Chen; Yuting Yang; Heya Luan; Shaoqi Li; Ruina Li; Boye Wen; Sirong Lv; Runqi Chen; Jinxuan Guo; Cuibai Wei

Stem Cell Research (Jun 2024)

Generation of human induced pluripotent stem cell line (XWHNi004-A) from a male with APOE gene mutation

Pin Wang,
Wenxian Sun,
Jin Gong,
Xiaodong Han,
Chang Xu,
Yufei Chen,
Yuting Yang,
Heya Luan,
Shaoqi Li,
Ruina Li,
Boye Wen,
Sirong Lv,
Runqi Chen,
Jinxuan Guo,
Cuibai Wei

Affiliations

Pin Wang: Innovation Center for Neurological Disorders and Department of Neurology, Xuanwu Hospital, Capital Medical University, National Clinical Research Center for Geriatric Diseases, Beijing, China
Wenxian Sun: Innovation Center for Neurological Disorders and Department of Neurology, Xuanwu Hospital, Capital Medical University, National Clinical Research Center for Geriatric Diseases, Beijing, China
Jin Gong: College of Integrated Traditional Chinese and Western Medicine, Changchun University of Chinese Medicine, Changchun, China
Xiaodong Han: Innovation Center for Neurological Disorders and Department of Neurology, Xuanwu Hospital, Capital Medical University, National Clinical Research Center for Geriatric Diseases, Beijing, China
Chang Xu: Innovation Center for Neurological Disorders and Department of Neurology, Xuanwu Hospital, Capital Medical University, National Clinical Research Center for Geriatric Diseases, Beijing, China
Yufei Chen: Innovation Center for Neurological Disorders and Department of Neurology, Xuanwu Hospital, Capital Medical University, National Clinical Research Center for Geriatric Diseases, Beijing, China
Yuting Yang: School of Biological Science and Medical Engineering, Beihang University, Beijing, China
Heya Luan: Innovation Center for Neurological Disorders and Department of Neurology, Xuanwu Hospital, Capital Medical University, National Clinical Research Center for Geriatric Diseases, Beijing, China
Shaoqi Li: College of Integrated Traditional Chinese and Western Medicine, Changchun University of Chinese Medicine, Changchun, China
Ruina Li: School of Biological Science and Medical Engineering, Beihang University, Beijing, China
Boye Wen: Innovation Center for Neurological Disorders and Department of Neurology, Xuanwu Hospital, Capital Medical University, National Clinical Research Center for Geriatric Diseases, Beijing, China
Sirong Lv: Innovation Center for Neurological Disorders and Department of Neurology, Xuanwu Hospital, Capital Medical University, National Clinical Research Center for Geriatric Diseases, Beijing, China
Runqi Chen: School of Biological Science and Medical Engineering, Beihang University, Beijing, China
Jinxuan Guo: College of Integrated Traditional Chinese and Western Medicine, Changchun University of Chinese Medicine, Changchun, China
Cuibai Wei: Innovation Center for Neurological Disorders and Department of Neurology, Xuanwu Hospital, Capital Medical University, National Clinical Research Center for Geriatric Diseases, Beijing, China; Corresponding author.

Journal volume & issue: Vol. 77
p. 103398

Abstract

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Genetic polymorphism of apolipoprotein E (APOE) confers differential susceptibility to Alzheimer's disease (AD), and APOE ɛ4 variants is the most powerful risk factor for this disease. Here, we report the generation of a human induced pluripotent stem cell (iPSC) line carrying the APOE ɛ4/ɛ4 genotype from peripheral blood mononuclear cells (PBMCs) isolated from a male with a family history of AD utilizing non-integrative Sendai virus vector. The iPSC maintains their original genotype, highly express endogenous pluripotency markers, displays a normal karyotype, and retains the ability to differentiate into cells representative of the three germ layers.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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