MicroRNA Targeting of CoREST Controls Polarization of Migrating Cortical Neurons

Marie-Laure Volvert; Pierre-Paul Prévot; Pierre Close; Sophie Laguesse; Sophie Pirotte; James Hemphill; Florence Rogister; Nathalie Kruzy; Rosalie Sacheli; Gustave Moonen; Alexander Deiters; Matthias Merkenschlager; Alain Chariot; Brigitte Malgrange; Juliette D. Godin; Laurent Nguyen

doi:10.1016/j.celrep.2014.03.075

Cell Reports (May 2014)

MicroRNA Targeting of CoREST Controls Polarization of Migrating Cortical Neurons

Marie-Laure Volvert,
Pierre-Paul Prévot,
Pierre Close,
Sophie Laguesse,
Sophie Pirotte,
James Hemphill,
Florence Rogister,
Nathalie Kruzy,
Rosalie Sacheli,
Gustave Moonen,
Alexander Deiters,
Matthias Merkenschlager,
Alain Chariot,
Brigitte Malgrange,
Juliette D. Godin,
Laurent Nguyen

Affiliations

Marie-Laure Volvert: GIGA-Neurosciences, University of Liège, C.H.U. Sart Tilman, Liège 4000, Belgium
Pierre-Paul Prévot: GIGA-Neurosciences, University of Liège, C.H.U. Sart Tilman, Liège 4000, Belgium
Pierre Close: GIGA-Signal Transduction, University of Liège, C.H.U. Sart Tilman, Liège 4000, Belgium
Sophie Laguesse: GIGA-Neurosciences, University of Liège, C.H.U. Sart Tilman, Liège 4000, Belgium
Sophie Pirotte: GIGA-Neurosciences, University of Liège, C.H.U. Sart Tilman, Liège 4000, Belgium
James Hemphill: Department of Chemistry, North Carolina State University, Raleigh, NC 27695, USA
Florence Rogister: GIGA-Neurosciences, University of Liège, C.H.U. Sart Tilman, Liège 4000, Belgium
Nathalie Kruzy: GIGA-Neurosciences, University of Liège, C.H.U. Sart Tilman, Liège 4000, Belgium
Rosalie Sacheli: GIGA-Neurosciences, University of Liège, C.H.U. Sart Tilman, Liège 4000, Belgium
Gustave Moonen: GIGA-Neurosciences, University of Liège, C.H.U. Sart Tilman, Liège 4000, Belgium
Alexander Deiters: Department of Chemistry, North Carolina State University, Raleigh, NC 27695, USA
Matthias Merkenschlager: Lymphocyte Development Group, MRC Clinical Sciences Centre, Imperial College London, Du Cane Road, London W12 0NN, UK
Alain Chariot: GIGA-Signal Transduction, University of Liège, C.H.U. Sart Tilman, Liège 4000, Belgium
Brigitte Malgrange: GIGA-Neurosciences, University of Liège, C.H.U. Sart Tilman, Liège 4000, Belgium
Juliette D. Godin: GIGA-Neurosciences, University of Liège, C.H.U. Sart Tilman, Liège 4000, Belgium
Laurent Nguyen: GIGA-Neurosciences, University of Liège, C.H.U. Sart Tilman, Liège 4000, Belgium

DOI: https://doi.org/10.1016/j.celrep.2014.03.075
Journal volume & issue: Vol. 7, no. 4
pp. 1168 – 1183

Abstract

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The migration of cortical projection neurons is a multistep process characterized by dynamic cell shape remodeling. The molecular basis of these changes remains elusive, and the present work describes how microRNAs (miRNAs) control neuronal polarization during radial migration. We show that miR-22 and miR-124 are expressed in the cortical wall where they target components of the CoREST/REST transcriptional repressor complex, thereby regulating doublecortin transcription in migrating neurons. This molecular pathway underlies radial migration by promoting dynamic multipolar-bipolar cell conversion at early phases of migration, and later stabilization of cell polarity to support locomotion on radial glia fibers. Thus, our work emphasizes key roles of some miRNAs that control radial migration during cerebral corticogenesis.

Published in Cell Reports

ISSN: 2211-1247 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: http://www.cell.com/cell-reports/home

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