International Journal of Nanomedicine (Sep 2018)
3D-printed scaffolds of mesoporous bioglass/gliadin/polycaprolactone ternary composite for enhancement of compressive strength, degradability, cell responses and new bone tissue ingrowth
Abstract
Yiqun Zhang,1 Wei Yu,1 Zhaoyu Ba,2 Shusen Cui,1 Jie Wei,3 Hong Li4 1Department of Hand Surgery, China-Japan Union Hospital, Jilin University, Changchun 130033, China; 2Department of Spinal Surgery, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, China; 3Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, China; 4College of Physical Science and Technology, Sichuan University, Chengdu 610041, China Background: Due to the increasing number of patients with bone defects, bone nonunion and osteomyelitis, tumor and congenital diseases, bone repair has become an urgent problem to be solved.Methods: In this study, the 3D-printed scaffolds of ternary composites containing mesoporous bioglass fibers of magnesium calcium silicate (mMCS), gliadin (GA) and polycaprolactone (PCL) were fabricated using a 3D Bioprinter.Results: The compressive strength and in vitro degradability of the mMCS/GA/PCL composites (MGPC) scaffolds were improved with the increase of mMCS content. In addition, the attachment and proliferation of MC3T3-E1 cells on the scaffolds were significantly promoted with the increase of mMCS content. Moreover, the cells with normal phenotype attached and spread well on the scaffolds surfaces, indicating good cytocompatibility. The scaffolds were implanted into the femur defects of rabbits, and the results demonstrated that the scaffold containing mMCS stimulated new bone formation and ingrowth into the scaffolds through scaffolds degradation in vivo. Moreover, the expression of type I collagen into scaffolds was enhanced with the increase of mMCS content.Conclusion: The 3D-printed MGPC scaffold with controllable architecture, good biocompatibility, high compressive strength, proper degradability and excellent in vivo osteogenesis has great potential for bone regeneration. Keywords: ternary composites, polymer-based composite, biocompatibility, cytocompatibility, osteogenesis
