TET enzymes regulate skeletal development through increasing chromatin accessibility of RUNX2 target genes

Lijun Wang; Xiuling You; Dengfeng Ruan; Rui Shao; Hai-Qiang Dai; Weiliang Shen; Guo-Liang Xu; Wanlu Liu; Weiguo Zou

doi:10.1038/s41467-022-32138-x

Nature Communications (Aug 2022)

TET enzymes regulate skeletal development through increasing chromatin accessibility of RUNX2 target genes

Lijun Wang,
Xiuling You,
Dengfeng Ruan,
Rui Shao,
Hai-Qiang Dai,
Weiliang Shen,
Guo-Liang Xu,
Wanlu Liu,
Weiguo Zou

Affiliations

Lijun Wang: Institute of Microsurgery on Extremities, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital
Xiuling You: State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, CAS Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of Sciences
Dengfeng Ruan: Department of Orthopedic Surgery, the Second Affiliated Hospital, Zhejiang University School of Medicine
Rui Shao: Institute of Microsurgery on Extremities, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital
Hai-Qiang Dai: State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, CAS Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of Sciences
Weiliang Shen: Department of Orthopedic Surgery, the Second Affiliated Hospital, Zhejiang University School of Medicine
Guo-Liang Xu: State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, CAS Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of Sciences
Wanlu Liu: Department of Orthopedic Surgery, the Second Affiliated Hospital, Zhejiang University School of Medicine
Weiguo Zou: Institute of Microsurgery on Extremities, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital

DOI: https://doi.org/10.1038/s41467-022-32138-x
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 15

Abstract

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Here the authors investigate the role of the TET family of DNA demethylases in mammalian skeletal development. They find that loss of TETs leads to hypermethylation that results in decreased chromatin accessibility of RUNX2 target genes, repressing osteoblast differentiation and leading to skeletal defects in mouse such as short limbs.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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