Inclusion of different exogenous fibrolytic enzymes to dry jowar fodder and their effect on in vitro total gas production

Abstract

Aim: Our objective was to estimate in-vitro gas production from dry jowar fodder added with differentconcentrations of exogenous fibrolytic enzymes (EFEs) like neutral cellulase and fungal xylanase.Materials and Methods: 34 different samples of dry jowar fodder were prepared according to differentconcentrations of neutral cellulase, fungal xylanase and neutral cellulase + fungal xylanase (1:1). Sample notcontaining any enzymes was considered as the control group. These 34 samples were subjected to further in vitrogas production analysis.Results: Statistically, significantly higher (P<0.05) potential gas production was recorded for 0.7 % at 6 hr period,0.7 % at 12 hr period, 0.7 %, 0.8 % at 18 hr period and 0.7 %, 0.8 % at 24 hr period in the samples treated withneutral cellulase. Significantly higher potential gas production was recorded for 0.5 %, 0.8 % at 6 hr period, 0.5 %,0.6 %, 0.8 % at 12 hr period, 0.8 % at 18 hr period and 0.5 %, 0.6 %, 0.8 % at 24 hr period in the samples treated withfungal xylanase. Significantly higher potential gas production was recorded for 0.6 %, 0.6 %, 0.8 % at 6 hr period,0.6 %, 0.8 % at 12 hr period, 0.6 %, 0.8 % at 18 hr period and 0.6 %, 0.8 % at 24 hr period in the samples treated withmixture of neutral cellulase + fungal xylanase (1:1).Conclusion: Addition of neutral cellulase and fungal xylanase into the samples of dry jowar fodder increased invitro total potential gas production. EFEs increase substrate degradation and there by improve the nutritive value ofdry jowar fodder.

Keywords

• dry jowar fodder
• fungal xylanase
• neutral cellulase
• total gas production