Unveiling the potential role of gibberellic acid, melatonin and indole acetic acid on parthenocarpy, physiological traits and phytochemical responses in Hibiscus sabdariffa L

Abstract

Abstract The economic part of the Hibiscus sabdariffa L. (Malvaceae) plant is the sepal. One of the main challenges in harvesting this product is separating its seeds, which are surrounded by sepals. If the parthenocarpy process occurs without seeds, the labor costs are reduced and the profits from the production of this plant are increased. In current study, the effect of gibberellic acid (GA3), melatonin (M), and indole acetic acid (IAA) on the induction of parthenocarpy in H. sabdariffa plants was investigated. The study was conducted as a factorial experiment in the form of randomized complete block design with three replications. Different concentrations of GA3 (0 (control), 700, 800, and 900 ppm), M (0 (control), 100, 200, and 400 ppm), and IAA (0 (control), 1000, 1200, and 1400 ppm) were foliar sprayed after the emergence of the flower bud on the 50th, 60th, and 70th days of planting, and the control plants were also sprayed with distilled water. Various measurements were taken including the number of seeds per boll, parthenocarpy percentage, capsule volume, number of bolls per plant, number of mature seeds, and phytochemical parameters such as anthocyanin, chlorophyll and carotenoid content, antioxidant value, total phenol and flavonoid content, and soluble solids. The application of GA3 at 800 and 900 ppm along with IAA at 1000 ppm and M at 100 and 200 ppm led to the production of parthenocarpy fruits and showed the best results in the induction of parthenocarpy. The control group indicated the highest number of mature seeds per boll. The highest amount of phenol and flavonoid contents were obtained in plants treated with GA3 at 800 ppm. For anthocyanin, the control group showed the highest value (1.63 mg g−1), and in the case of the antioxidant trait, plants exposed to 100 ppm M showed the highest IC50 (40.68%). Hence, the application of plant growth regulators with appropriate concentrations can be effective in inducing parthenocarpy in H. sabdariffa plants. Additionally, parthenocarpy-induced by GA3, IAA, and M had different impacts on fruit quality and quantity, suggesting that the effect depends on the type of employed hormones used and their concentrations.

Keywords

• Parthenocarpy
• Hibiscus sabdariffa
• Growth regulators
• Foliar application