Haematologica (Sep 2010)

The majority of the in vitro erythroid expansion potential resides in CD34− cells, outweighing the contribution of CD34+ cells and significantly increasing the erythroblast yield from peripheral blood samples

  • Emile van den Akker,
  • Timothy J. Satchwell,
  • Stephanie Pellegrin,
  • Geoff Daniels,
  • Ashley M. Toye

DOI
https://doi.org/10.3324/haematol.2009.019828
Journal volume & issue
Vol. 95, no. 9

Abstract

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The study of human erythropoiesis in health and disease requires a robust culture system that consistently and reliably generates large numbers of immature erythroblasts that can be induced to differentiate synchronously. We describe a culture method modified from Leberbauer et al. (2005) and obtain a homogenous population of erythroblasts from peripheral blood mononuclear cells (PBMC) without prior purification of CD34+ cells. This pure population of immature erythroblasts can be expanded to obtain 4×108 erythroblasts from 1×108 PBMC after 13–14 days in culture. Upon synchronized differentiation, high levels of enucleation (80–90%) and low levels of cell death (