Paper-Based Biosensors for the Detection of Nucleic Acids from Pathogens

Jiangshan Wang; Josiah Levi Davidson; Simerdeep Kaur; Andres A. Dextre; Mohsen Ranjbaran; Mohamed S. Kamel; Shreya Milind Athalye; Mohit S. Verma

doi:10.3390/bios12121094

Biosensors (Nov 2022)

Paper-Based Biosensors for the Detection of Nucleic Acids from Pathogens

Jiangshan Wang,
Josiah Levi Davidson,
Simerdeep Kaur,
Andres A. Dextre,
Mohsen Ranjbaran,
Mohamed S. Kamel,
Shreya Milind Athalye,
Mohit S. Verma

Affiliations

Jiangshan Wang: Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN 47907, USA
Josiah Levi Davidson: Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN 47907, USA
Simerdeep Kaur: Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN 47907, USA
Andres A. Dextre: Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN 47907, USA
Mohsen Ranjbaran: Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN 47907, USA
Mohamed S. Kamel: Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN 47907, USA
Shreya Milind Athalye: Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN 47907, USA
Mohit S. Verma: Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN 47907, USA

DOI: https://doi.org/10.3390/bios12121094
Journal volume & issue: Vol. 12, no. 12
p. 1094

Abstract

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Paper-based biosensors are microfluidic analytical devices used for the detection of biochemical substances. The unique properties of paper-based biosensors, including low cost, portability, disposability, and ease of use, make them an excellent tool for point-of-care testing. Among all analyte detection methods, nucleic acid-based pathogen detection offers versatility due to the ease of nucleic acid synthesis. In a point-of-care testing context, the combination of nucleic acid detection and a paper-based platform allows for accurate detection. This review offers an overview of contemporary paper-based biosensors for detecting nucleic acids from pathogens. The methods and limitations of implementing an integrated portable paper-based platform are discussed. The review concludes with potential directions for future research in the development of paper-based biosensors.

Published in Biosensors

ISSN: 2079-6374 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Technology: Chemical technology: Biotechnology
Website: http://www.mdpi.com/journal/biosensors

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