EXPERIENCE OF USING THE MINIMUM INHIBITORY CONCENTRATION METHOD ON LIQUID NUTRITIONAL MEDIA FOR DRUG SUSCEPTIBILITY TESTING OF NON-TUBERCULOUS MYCOBACTERIA

Abstract

Goal of the study: analysis of drug resistance of certain species of non-tuberculous mycobacteria through testing by minimum inhibitory concentrations on the fluid nutritional media.Materials and method: drug resistance of non-tuberculous mycobacteria was tested by the method of minimum inhibitory concentrations on the fluid nutritional media. 51 strains of non-tuberculous mycobacteria from the species of M. intracellulare, M. avium, M. kansasii, M. gordonae, M. fortuitum, M. abscessus were tested. The minimum inhibitory concentration of the drug for slow-growing non-tuberculous mycobacteria was defined using the panel of 13 drugs (SLOWMYCO) and for the fast-growing mycobacteria – the panel of 15 drugs RAPMYCO Sensititre (Trek Diagnostic System, Thermo Scientific, USA) was used. It was proved that the majority of tested fast growing mycobacteria were resistant to ceftriaxone, cefepime, cefoxitin but susceptible to amikacin; and slow growing non-tuberculous mycobacteria were susceptible to clarithromycin, linezolid and amikacin.

Keywords

• лекарственная устойчивость
• нетуберкулезные микобактерии
• минимальная ингибирующая концентрация