Generation of iPSC from cardiac and tail-tip fibroblasts derived from a second heart field reporter mouse

Javier Linares; Estibaliz Arellano-Viera; Olalla Iglesias-García; Carmen Ferreira; Elena Iglesias; Gloria Abizanda; Felipe Prósper; Xonia Carvajal-Vergara

doi:10.1016/j.scr.2016.03.006

Stem Cell Research (May 2016)

Generation of iPSC from cardiac and tail-tip fibroblasts derived from a second heart field reporter mouse

Javier Linares,
Estibaliz Arellano-Viera,
Olalla Iglesias-García,
Carmen Ferreira,
Elena Iglesias,
Gloria Abizanda,
Felipe Prósper,
Xonia Carvajal-Vergara

Affiliations

Javier Linares: Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Instituto de Investigación Sanitaria de Navarra, Pamplona, Spain
Estibaliz Arellano-Viera: Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Instituto de Investigación Sanitaria de Navarra, Pamplona, Spain
Olalla Iglesias-García: Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Instituto de Investigación Sanitaria de Navarra, Pamplona, Spain
Carmen Ferreira: Genetic Analysis Core Facility, University of Navarra, Instituto de Investigación Sanitaria de Navarra, Pamplona, Spain
Elena Iglesias: Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Instituto de Investigación Sanitaria de Navarra, Pamplona, Spain
Gloria Abizanda: Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Instituto de Investigación Sanitaria de Navarra, Pamplona, Spain
Felipe Prósper: Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Instituto de Investigación Sanitaria de Navarra, Pamplona, Spain
Xonia Carvajal-Vergara: Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Instituto de Investigación Sanitaria de Navarra, Pamplona, Spain

DOI: https://doi.org/10.1016/j.scr.2016.03.006
Journal volume & issue: Vol. 16, no. 3
pp. 617 – 621

Abstract

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Mef2c Anterior Heart Field (AHF) enhancer is activated during embryonic heart development and it is expressed in multipotent cardiovascular progenitors (CVP) giving rise to endothelial and myocardial components of the outflow tract, right ventricle and ventricular septum. Here we have generated iPSC from transgenic Mef2c-AHF-Cre x Ai6(RCLZsGreen) mice. These iPSC will provide a novel tool to investigate the AHF-CVP and their cell progeny.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

About the journal