Optimized workflow for human PBMC multiomic immunosurveillance studies

Abstract

Summary: Deep immune profiling is essential for understanding the human immune system in health and disease. Successful biological interpretation of this data requires consistent laboratory processing with minimal batch-to-batch variation. Here, we detail a robust pipeline for the profiling of human peripheral blood mononuclear cells by both high-dimensional flow cytometry and single-cell RNA-seq. These protocols reduce batch effects, generate reproducible data, and increase throughput.For complete details on the use and execution of this protocol, please refer to Savage et al. (2021).

Keywords

• Cell Biology
• Single Cell
• Flow Cytometry/Mass Cytometry
• Sequencing
• RNAseq
• Immunology