Nature Communications (Jul 2022)
Massively targeted evaluation of therapeutic CRISPR off-targets in cells
- Xiaoguang Pan,
- Kunli Qu,
- Hao Yuan,
- Xi Xiang,
- Christian Anthon,
- Liubov Pashkova,
- Xue Liang,
- Peng Han,
- Giulia I. Corsi,
- Fengping Xu,
- Ping Liu,
- Jiayan Zhong,
- Yan Zhou,
- Tao Ma,
- Hui Jiang,
- Junnian Liu,
- Jian Wang,
- Niels Jessen,
- Lars Bolund,
- Huanming Yang,
- Xun Xu,
- George M. Church,
- Jan Gorodkin,
- Lin Lin,
- Yonglun Luo
Affiliations
- Xiaoguang Pan
- Lars Bolund Institute of Regenerative Medicine, Qingdao-Europe Advanced Institute for Life Sciences, BGI-Qingdao, BGI-Shenzhen
- Kunli Qu
- Lars Bolund Institute of Regenerative Medicine, Qingdao-Europe Advanced Institute for Life Sciences, BGI-Qingdao, BGI-Shenzhen
- Hao Yuan
- Lars Bolund Institute of Regenerative Medicine, Qingdao-Europe Advanced Institute for Life Sciences, BGI-Qingdao, BGI-Shenzhen
- Xi Xiang
- Lars Bolund Institute of Regenerative Medicine, Qingdao-Europe Advanced Institute for Life Sciences, BGI-Qingdao, BGI-Shenzhen
- Christian Anthon
- Center for non-coding RNA in Technology and Health, Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen
- Liubov Pashkova
- Center for non-coding RNA in Technology and Health, Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen
- Xue Liang
- Lars Bolund Institute of Regenerative Medicine, Qingdao-Europe Advanced Institute for Life Sciences, BGI-Qingdao, BGI-Shenzhen
- Peng Han
- Lars Bolund Institute of Regenerative Medicine, Qingdao-Europe Advanced Institute for Life Sciences, BGI-Qingdao, BGI-Shenzhen
- Giulia I. Corsi
- Center for non-coding RNA in Technology and Health, Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen
- Fengping Xu
- Lars Bolund Institute of Regenerative Medicine, Qingdao-Europe Advanced Institute for Life Sciences, BGI-Qingdao, BGI-Shenzhen
- Ping Liu
- BGI-Research, BGI-Shenzhen
- Jiayan Zhong
- BGI-Research, BGI-Shenzhen
- Yan Zhou
- Department of Biomedicine, Aarhus University
- Tao Ma
- BGI-Research, BGI-Shenzhen
- Hui Jiang
- BGI-Research, BGI-Shenzhen
- Junnian Liu
- Lars Bolund Institute of Regenerative Medicine, Qingdao-Europe Advanced Institute for Life Sciences, BGI-Qingdao, BGI-Shenzhen
- Jian Wang
- BGI-Research, BGI-Shenzhen
- Niels Jessen
- Department of Biomedicine, Aarhus University
- Lars Bolund
- Lars Bolund Institute of Regenerative Medicine, Qingdao-Europe Advanced Institute for Life Sciences, BGI-Qingdao, BGI-Shenzhen
- Huanming Yang
- BGI-Research, BGI-Shenzhen
- Xun Xu
- BGI-Research, BGI-Shenzhen
- George M. Church
- Department of Genetics, Blavatnik Institute, Harvard Medical School
- Jan Gorodkin
- Center for non-coding RNA in Technology and Health, Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen
- Lin Lin
- Department of Biomedicine, Aarhus University
- Yonglun Luo
- Lars Bolund Institute of Regenerative Medicine, Qingdao-Europe Advanced Institute for Life Sciences, BGI-Qingdao, BGI-Shenzhen
- DOI
- https://doi.org/10.1038/s41467-022-31543-6
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 14
Abstract
Thorough evaluation of CRISPR RNA-guided nucleases off-targets in cells is required for advancing gene therapies. Here the authors report SURRO-seq for the simultaneous investigation of thousands of off-target sites for therapeutic RNA-guided nucleases in cells.
