Acta Dermato-Venereologica (Nov 2021)

Differential Immunoexpression of BRAF/V600E, Senescence Markers, PTEN, and T-type Calcium Channels in Acquired Naevi According to their Histopathological and Dermoscopic Classification

  • Sara Moreno,
  • Oscar Maiques,
  • Carla Barcelo,
  • Marta Romero,
  • Maria Santacana,
  • Ignacio Gómez,
  • Dolors Cuevas,
  • Ana Velasco,
  • Alvar Vea,
  • Anna Macia,
  • Ramon Boix,
  • Joan Valls,
  • Sonia Gatius,
  • Carles Canti,
  • Xavier Matias-Guiu,
  • Xavier Soria,
  • Rosa M. Marti

DOI
https://doi.org/10.2340/actadv.v101.361
Journal volume & issue
Vol. 101, no. 11

Abstract

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BRAF/V600E mutation and other cell growth/growth-control mechanisms are involved in naevogenesis and melanomagenesis. Immunoexpression of BRAF/V600E and other molecules (p16, phosphatase and tensin homologue (PTEN), Ki67, hTERT and Cav3.1 and 3.2 calcium channels) were investigated in 80 histopatho­logically and dermoscopically classified acquired naevi. Regarding BRAF/V600E, dysplastic naevi showed lower immunostaining than common naevi, which was significant in comparison with intradermal naevi, which showed the highest BRAF/V600E histoscore. Junctional naevi showed the lowest BRAF/V600E levels. Globular/cobblestone and reticular dermoscopic patterns were consistently associated with high and low BRAF/V600E immunoexpression, respectively, but Zalaudek’s peripheral globule pattern (CR/PG) showed the highest BRAF/V600E immunoexpression. Among global patterns, the previously not investigated multicomponent pattern showed the lowest BRAF/V600E immunoexpression. Regarding the remaining biomarkers, new immunohistochemical features were found, in particular p16 and PTEN low expression in multicomponent pattern; and Ki67, hTERT and Cav.3.1 high expression in CR/PG. In conclusion, histopathology and dermoscopy provide complementary information regarding the biology of melanocytic naevi.

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