G3: Genes, Genomes, Genetics (Sep 2020)

A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions

  • Dae-Kyum Kim,
  • Jennifer J. Knapp,
  • Da Kuang,
  • Aditya Chawla,
  • Patricia Cassonnet,
  • Hunsang Lee,
  • Dayag Sheykhkarimli,
  • Payman Samavarchi-Tehrani,
  • Hala Abdouni,
  • Ashyad Rayhan,
  • Roujia Li,
  • Oxana Pogoutse,
  • Étienne Coyaud,
  • Sylvie van der Werf,
  • Caroline Demeret,
  • Anne-Claude Gingras,
  • Mikko Taipale,
  • Brian Raught,
  • Yves Jacob,
  • Frederick P. Roth

DOI
https://doi.org/10.1534/g3.120.401554
Journal volume & issue
Vol. 10, no. 9
pp. 3399 – 3402

Abstract

Read online

The world is facing a global pandemic of COVID-19 caused by the SARS-CoV-2 coronavirus. Here we describe a collection of codon-optimized coding sequences for SARS-CoV-2 cloned into Gateway-compatible entry vectors, which enable rapid transfer into a variety of expression and tagging vectors. The collection is freely available. We hope that widespread availability of this SARS-CoV-2 resource will enable many subsequent molecular studies to better understand the viral life cycle and how to block it.

Keywords