Revista Colombiana de Biotecnología (Feb 2008)
Micropropagation of Dodonea viscosa (L) Jacq: an endangered plant
Abstract
An efficient in vitro propagation system was developed for Dodonea viscosa (L) Jacq., this being an endangered endemic shrub. This study was aimed at evaluating the effect of four growth regulators on the in vitro morphogenetic development of nodes and leaves. The basic culture medium used was Murashige and Skoog (1962), enriched with the following growth regulators: butyric indol acid and benzylaminopurine (0, 0.5, 1 and 1.5 mg.L-1) in node culture and 2, 4-dichlorofenoxy acetic acid and kinetin (0, 1, 3 and 5 mg/l-1) in leaves. D. viscosa has been propagated in in vitro conditions by using 2% benlate for one hour and 0.5% sodium hypochlorite for 5 min. Incubation in the dark has controlled D. viscosa explant oxidation, whereas using citric acid and ascorbic acid has not controlled the oxidation of explants exposed to light. The node is a suitable explant for micro- propagating D. viscous in the absence of phytohormones. The best morphogenetic-inducing treatment of leaves was the eighth one assayed (1.0 mg/l-1 kinetin and 5.0 mg/l-1 2,4-D). This protocol represents a conservation model for endangered species and encourages large-scale Dodonea viscosa (L) Jacq. propagation.
