Jurnal Veteriner (May 2014)
Fourier Transform Infrared Sebagai Metode Alternatif Penetapan Tingkat Stres pada Sapi (FOURIER TRANSFORM INFRARED AS AN ALTERNATIVE TOOL FOR DETERMINING OF STRESS IN COW)
Abstract
Stress in animal is a condition of nonspesific discomfort which cause of non-specific immune defects,failure of reproduction, and decreased of meat carcass until the death of animals. To determine stress ofcattle, it will be invented stress detector using a non-invasive method based on the spectroscopy FourierTransform Infrared (FTIR). Basically, FTIR will detect component in compound of cathecolamine andcortisol as ketone (= O) and methyl (= CH 3). Furthermore, each group of components will be detected indifferent of absorbant and wavelength. The results showed that average level of cortisol in female beefcattle durimg resting eriod was 38,48±21,53 ng/dL, on time of slaughtering were 116,88±112,59 ng/dL. Forbull, which were resting 20,42±9,25 ng/dL; when animal was slaughtered level of cortisol was 67,61±41,62ng/dL. Using FTIR, it was showed that compound of metil was absorbed well. Animals with udder stresscondition have been recorded on the wave lenght of 2777-3456 nm. It has been concluded level of cortisolon cattle which were resting is significantly different from animal which were slaughtering P(< 0.05),where cortisol would increase drastically. Using Calibration of FTIR indicated resting animals only havefewer value of absorbance than animals which slaughtered. FTIR is a very prospect method for makingstress indicator.
