Seed germination and development of orchid seedlings (Cyrtopodium saintlegerianum) with fungi

Abstract

Abstract Fungi of Rhizoctonia complex are mycorrhizal of orchids and may to germinate yours seeds and development the seedlings. In this context, our objective was to select a fungal isolate to promote seed germination and seedling development of Cyrtopodium saintlegerianum. Pelotons were found in the roots and three mycorrhizal fungi were isolated. We tested mycorrhizal isolates obtained from C. saintlegerianum roots and six mycorrhizal fungi from other orchids as well three pathogenic isolates (of rice and bean) to germinate the seeds in oatmeal-agar medium. Seeds not inoculated were used as control. The isolates En07 (Waitea circinata), Cs10 (Tulasnella sp.) and Ro88 (Rhizoctonia oryzae) were efficient to promote seed germination, but only En07 differing statistically of the control. The non-specific isolate En07 promoted germination in 81% of seeds and the specific isolate (Cs10) promoted 60%, evidencing the non-specificity mycorrhizal association in this orchid during germination. Axenic seedlings were inoculated with four mycorrhizal fungi (non-inoculated seedlings - control). After six months, the isolates En07 and Cs10 were efficient in the interaction with the seedlings, but did not differ to the control. Therefore, our results suggested that fungi of the Rhizoctonia complex can be used in the germination and seedling development of C. saintlegerianum.

Keywords

• associação micorrízica
• fungos não específicos
• Orchidaceae
• patógenos de plantas
• complexo Rhizoctonia