Cryo-EM structure of a licensed DNA replication origin

Ferdos Abid Ali; Max E. Douglas; Julia Locke; Valerie E. Pye; Andrea Nans; John F. X. Diffley; Alessandro Costa

doi:10.1038/s41467-017-02389-0

Nature Communications (Dec 2017)

Cryo-EM structure of a licensed DNA replication origin

Ferdos Abid Ali,
Max E. Douglas,
Julia Locke,
Valerie E. Pye,
Andrea Nans,
John F. X. Diffley,
Alessandro Costa

Affiliations

Ferdos Abid Ali: Macromolecular Machines Laboratory, The Francis Crick Institute
Max E. Douglas: Chromosome Replication Laboratory, The Francis Crick Institute
Julia Locke: Macromolecular Machines Laboratory, The Francis Crick Institute
Valerie E. Pye: Chromatin Structure and Mobile DNA Laboratory, The Francis Crick Institute
Andrea Nans: Structural Biology of Cells and Viruses, The Francis Crick Institute
John F. X. Diffley: Chromosome Replication Laboratory, The Francis Crick Institute
Alessandro Costa: Macromolecular Machines Laboratory, The Francis Crick Institute

DOI: https://doi.org/10.1038/s41467-017-02389-0
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 10

Abstract

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Origins of replication are licensed by loading of MCM onto DNA, and origin firing depends on interaction with Cdc45 and GINS to form two CMG holo-helicases. Here, authors determine the cryo-EM structures of DNA-bound MCM and visualise a phospho-dependent MCM element important for Cdc45 recruitment.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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