Unraveling the determinants of microRNA mediated regulation using a massively parallel reporter assay

Ilya Vainberg Slutskin; Shira Weingarten-Gabbay; Ronit Nir; Adina Weinberger; Eran Segal

doi:10.1038/s41467-018-02980-z

Nature Communications (Feb 2018)

Unraveling the determinants of microRNA mediated regulation using a massively parallel reporter assay

Ilya Vainberg Slutskin,
Shira Weingarten-Gabbay,
Ronit Nir,
Adina Weinberger,
Eran Segal

Affiliations

Ilya Vainberg Slutskin: Department of Computer Science and Applied Mathematics, Weizmann Institute of Science
Shira Weingarten-Gabbay: Department of Computer Science and Applied Mathematics, Weizmann Institute of Science
Ronit Nir: Department of Computer Science and Applied Mathematics, Weizmann Institute of Science
Adina Weinberger: Department of Computer Science and Applied Mathematics, Weizmann Institute of Science
Eran Segal: Department of Computer Science and Applied Mathematics, Weizmann Institute of Science

DOI: https://doi.org/10.1038/s41467-018-02980-z
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 18

Abstract

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MiRNAs are known regulators of gene expression. Here the authors perform a large-scale massively parallel reporter assay to investigate the effect of a large number of designed 3′ UTR sequences on reporter expression and asses how miRNA regulatory elements features affect miRNA mediated repression.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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