ASSESSMENT OF THE HOMOLOGY OF HOG1, 14-3-3 AND STE11 IN REPRESENTATIVES OF SYMBIOTIC MYCORRHIZAL FUNGI

Abstract

Adaptation to drought of woody and shrubby plants is associated with species features/characteristics and the presence of symbiotic relationships with arbuscular mycorrhiza. There is a wide variety of arbuscular mycorrhiza in nature, the most widespread genera are: Glomus, Diversispora, Paraglomus, Acaulospora, Entrophospora, Gigaspora, Scutellospora. The signaling proteins Hog1, 14-3-3, Ste11 in arbuscular mycorrhiza fungi play an important role in triggering mechanisms of adaptation to stress in the symbiotic relationship "fungus-plant". In this regard, the goal of the work was to evaluate the homology of amino acid sequences of the signaling proteins Hog1, 14-3-3 and Ste11 in different symbiotic mycorrhizal fungi. For the study, their amino acid sequences were taken from the Protein database (NCBI) using the BLAST program, a search for protein homologues was performed, subsequently multiple alignment was performed by the MEGA11 program using the MUSCLE algorithm. Phylogenetic analysis was carried out in the MEGA11 program using the maximum likelihood method. As a result, high conservativity for the Hog1, 14-3-3 proteins and high variability for Ste11 were revealed. The divergence of a pair of homologues of the protein 14-3-3 in Glomeromycotina before the separation of the subphylum and Ste11 in Glomeraceae after the formation of the family was also shown. As a result, we determined that Ste11 is a promising protein of interest for searching for intraspecific variations associated with resistance to drought and other abiotic stress factors in the mushroom-plant system.

Keywords

• hog1
• 14-3-3
• ste11
• арбускулярная микориза
• множественное выравнивание
• филогенетика
• опустынивание
• hog 1
• 14-3-3
• ste11
• arbuscular mycorrhiza
• multiple alignment
• phylogenetics
• desertification