Lipe gene polymorphism c.442 g>a influence on carcass traits in pigs

Abstract

Hormone sensitive lipase is one of three enzymes involved in lipolysis process and encoded by LIPE gene. In this study we investigated LIPE gene polymorphism c.442 G>A influence on carcass traits in hybrid pigs. Genomic DNA extracted using Chelex resin, genotypes determined using RFLP-PCR. Allele A observed with frequency 0,738, allele G - 0,262. The most common genotype was AA, genotype GG was observed with lower frequency, genotype AG was rarest. While evaluating population heterozygosity, it was noticed that observed heterozygosity was only 0,075, while expected heterozygosity was 0,387. In observed pig population allele A is associated with better animal muscularity, allele G - with greater fat content.

Keywords

• LIPE gene
• SNP
• polymorphism
• c.442 G>A
• carcass traits