Nature Communications (May 2022)

PlasmidMaker is a versatile, automated, and high throughput end-to-end platform for plasmid construction

  • Behnam Enghiad,
  • Pu Xue,
  • Nilmani Singh,
  • Aashutosh Girish Boob,
  • Chengyou Shi,
  • Vassily Andrew Petrov,
  • Roy Liu,
  • Siddhartha Suryanarayana Peri,
  • Stephan Thomas Lane,
  • Emily Danielle Gaither,
  • Huimin Zhao

DOI
https://doi.org/10.1038/s41467-022-30355-y
Journal volume & issue
Vol. 13, no. 1
pp. 1 – 13

Abstract

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Despite their broad utility, design and construction of plasmids remains laborious and time-consuming. Here the authors report a robust, versatile, and automated end-to-end platform that enables scarless construction of virtually any plasmid.