Global mapping of GalNAc-T isoform-specificities and O-glycosylation site-occupancy in a tissue-forming human cell line

Mathias I. Nielsen; Noortje de Haan; Weston Kightlinger; Zilu Ye; Sally Dabelsteen; Minyan Li; Michael C. Jewett; Ieva Bagdonaite; Sergey Y. Vakhrushev; Hans H. Wandall

doi:10.1038/s41467-022-33806-8

Nature Communications (Oct 2022)

Global mapping of GalNAc-T isoform-specificities and O-glycosylation site-occupancy in a tissue-forming human cell line

Mathias I. Nielsen,
Noortje de Haan,
Weston Kightlinger,
Zilu Ye,
Sally Dabelsteen,
Minyan Li,
Michael C. Jewett,
Ieva Bagdonaite,
Sergey Y. Vakhrushev,
Hans H. Wandall

Affiliations

Mathias I. Nielsen: Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine, Faculty of Health and Medical Sciences, University of Copenhagen
Noortje de Haan: Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine, Faculty of Health and Medical Sciences, University of Copenhagen
Weston Kightlinger: Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine, Faculty of Health and Medical Sciences, University of Copenhagen
Zilu Ye: Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine, Faculty of Health and Medical Sciences, University of Copenhagen
Sally Dabelsteen: Department of Oral Medicine and Pathology, School of Dentistry, University of Copenhagen
Minyan Li: Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine, Faculty of Health and Medical Sciences, University of Copenhagen
Michael C. Jewett: Department of Chemical and Biological Engineering and Center for Synthetic Biology, Northwestern University
Ieva Bagdonaite: Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine, Faculty of Health and Medical Sciences, University of Copenhagen
Sergey Y. Vakhrushev: Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine, Faculty of Health and Medical Sciences, University of Copenhagen
Hans H. Wandall: Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine, Faculty of Health and Medical Sciences, University of Copenhagen

DOI: https://doi.org/10.1038/s41467-022-33806-8
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 17

Abstract

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Information about O-glycosylation site regulation and occupancy in the human proteome is limited. Here, the authors identify GalNAc transferase-specific glycan sites in human keratinocytes and describe their occupancy.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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