Construction of novel bienzyme-inorganic hybrid nanoflowers beads and their application in the efficient degradation of acridine

Abstract

To develop a biocatalyst with high efficiency, stability and reusability for the degradation of organic pollutant acridine in wastewater, a novel biocatalyst named bienzyme-inorganic hybrid nanoflowers beads were successfully prepared in the present work, designated as biE-HNFs-beads. This bienzyme biocatalyst composed of bienzyme-inorganic hybrid nanoflowers and traditional beads carriers were characterized by SEM, TEM, FT-IR, XRD, and EPR. The biE-HNFs-beads showed better stability, recyclability and improved catalytic activity for acridine degradation than that of free bienzyme. Furthermore, biE-HNFs-beads could reduce the diffusion and decomposition of the intermediate H2O2 through the bienzyme cascade reactions, which not only improved the stability of the bienzyme, but also accelerated the degradation of acridine. Under optimized conditions, the degradation rate of acridine by biE-HNFs-beads could reach 99.9 %. The stability study indicated that the biE-HNFs-beads exhibited enhanced stability, reusability and storage stability. Besides, the bioconversion products analysis of acridine degraded by biE-HNFs-beads biocatalyst were identified by LC-MS, and the enzymatic degradation pathway of acridine was inferred for the first time.

Keywords

• Biocatalysis
• Cascade reaction
• Acridine degradation
• Wastewater treatment
• Bienzyme-inorganic hybrid nanoflowers beads;