<i>Laetiporus sulphureus</i> Affects Migration and Superoxide Anion Radical Levels in HeLa Cervical Cancer Cells

Abstract

Cervical cancer is the fourth most common female malignancy worldwide. The treatment of cancer cells with metastatic potential is an important issue in cancer therapy. In this study, we investigate the edible and medicinal mushroom Laetiporus sulphureus (Bull.) Murrill, which has known biological properties for human health. Two selected concentrations (10 and 50 µg/mL) of L. sulphureus ethanolic extract were used to determine the levels of the superoxide anion radical (NBT test) and migratory potential (Wound healing test) on a cervical cancer cell line (HeLa). The effects were measured after 24 and 72 h. The extract induced an acute pro-oxidative effect on HeLa cells, with a significant reduction in the migratory potential of these cells in both tested concentrations. A higher concentration (50 µg/mL) had a slightly stronger antimigratory activity. Laetiporus sulphureus is a very important source of biologically active substances and should be reconsidered for the development of promising anticancer therapeutics.

Keywords

• cervical cancer
• wound healing
• fungotherapy
• NBT assay