Distinct expression profiles and overlapping functions of IL-4/13A and IL-4/13B in grass carp (Ctenopharyngodon idella)

Xinyu Jiang; Junya Wang; Shuai Wan; Yujie Xue; Zhaosheng Sun; Xingxing Cheng; Qian Gao; Jun Zou

Aquaculture and Fisheries (Mar 2020)

Distinct expression profiles and overlapping functions of IL-4/13A and IL-4/13B in grass carp (Ctenopharyngodon idella)

Xinyu Jiang,
Junya Wang,
Shuai Wan,
Yujie Xue,
Zhaosheng Sun,
Xingxing Cheng,
Qian Gao,
Jun Zou

Affiliations

Xinyu Jiang: Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China; International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China
Junya Wang: Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China; International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China
Shuai Wan: Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China; International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China
Yujie Xue: Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China; International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China
Zhaosheng Sun: Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China; International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China
Xingxing Cheng: Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China; International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China
Qian Gao: Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China; International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China; Corresponding author. Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China.
Jun Zou: Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China; International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China; Corresponding author. Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China.

Journal volume & issue: Vol. 5, no. 2
pp. 72 – 79

Abstract

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In mammals, interleukin (IL)-4 and IL-13 are important cytokines involved in the immune response of the host defence against pathogens and play critical roles in the polarization of monocytes/macrophages (MO/Mϕs). However, in teleost fish, the role of IL-4/13 homologues in the polarization of MO/Mϕ remains unknown. In this paper, we identified a second isoform of IL-4/13 (CiIL-4/13A) from grass carp (Ctenopharyngodon idella) in addition to the IL-4/13 homologue previously reported and comparatively studied their expression profiles and functions. The two CiIL-4/13 transcripts were constitutively expressed in tissues but displayed distinct expression patterns. Infection with Flavobacterium columnare or grass carp reovirus II (GCRV-Ⅱ) resulted in the alteration of expression levels of both IL-4/13 isoforms. Furthermore, in the primary head kidney cells, the recombinant CiIL-4/13A and B could up-regulate the expression of IL-10, inducible nitric oxide synthase (iNOS) and M2 macrophage marker gene (KLF4, kruppel like factor 4), whilst down-regulate the expression of IL-1β and M1 macrophage marker gene (MARCO, macrophage receptor with collagenous structure). The results suggest that the CiIL-4/13A and B play similar roles in regulating inflammation and differentiation of MO/Mϕs in grass carp. Keywords: Interleukin 4/13, Cytokine, Macrophage, Bacterial, Grass carp and viral infection

Published in Aquaculture and Fisheries

ISSN: 2468-550X (Online)
Publisher: KeAi Communications Co., Ltd.
Country of publisher: China
LCC subjects: Agriculture: Aquaculture. Fisheries. Angling
Website: https://www.keaipublishing.com/en/journals/aquaculture-and-fisheries

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