Non-viral derivation of a transgene-free induced pluripotent stem cell line from a male beagle dog

Sho Yoshimatsu; Kazuya Edamura; Yumi Yoshii; Aozora Iguchi; Hirotaka Kondo; Hisashi Shibuya; Tsukika Sato; Seiji Shiozawa; Hideyuki Okano

Stem Cell Research (May 2021)

Non-viral derivation of a transgene-free induced pluripotent stem cell line from a male beagle dog

Sho Yoshimatsu,
Kazuya Edamura,
Yumi Yoshii,
Aozora Iguchi,
Hirotaka Kondo,
Hisashi Shibuya,
Tsukika Sato,
Seiji Shiozawa,
Hideyuki Okano

Affiliations

Sho Yoshimatsu: Department of Physiology, School of Medicine, Keio University, Tokyo, Japan
Kazuya Edamura: Laboratory of Veterinary Surgery, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, Kanagawa, Japan
Yumi Yoshii: Laboratory of Veterinary Surgery, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, Kanagawa, Japan
Aozora Iguchi: Laboratory of Veterinary Surgery, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, Kanagawa, Japan
Hirotaka Kondo: Laboratory of Veterinary Pathology, College of Bioresource Sciences, Nihon University, Kanagawa, Japan
Hisashi Shibuya: Laboratory of Veterinary Pathology, College of Bioresource Sciences, Nihon University, Kanagawa, Japan
Tsukika Sato: Department of Physiology, School of Medicine, Keio University, Tokyo, Japan
Seiji Shiozawa: Department of Physiology, School of Medicine, Keio University, Tokyo, Japan
Hideyuki Okano: Department of Physiology, School of Medicine, Keio University, Tokyo, Japan; Corresponding author at: Department of Physiology, School of Medicine, Keio University, Shinjuku, Tokyo, Japan.

Journal volume & issue: Vol. 53
p. 102375

Abstract

Read online

We previously reported the non-viral derivation of transgene-free induced pluripotent stem cells (iPSCs) from somatic fibroblasts of a female beagle dog using an optimized induction medium and integration-free episomal vectors. Here, we report novel derivation of a male canine iPSC line OF35Y-iPS, which showed standard characteristics of pluripotency such as a strong gene expression profile of pluripotency markers, differentiation potential into all three germ layers, and normal karyotype (78XY). Furthermore, we demonstrated targeted integration of 2A-EGFP into the canine NANOS3 locus. The novel iPSC line would be a useful resource for stem cell research and regenerative veterinary medicine.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

About the journal