A novel fluorescent structure-switching aptasensor for the sensitive detection of acrylamide based on AuNPs-assisted separation of ssDNA

Ningyi Xu; Xinyue Ma; Yue Cao; Huan Wang; Han Wu; Hongru Zheng; Chuanyu Yang; Ruiqi Zou; Jiayi Liu; Chunyan Sun; Yuan Yuan

Advanced Agrochem (Sep 2023)

A novel fluorescent structure-switching aptasensor for the sensitive detection of acrylamide based on AuNPs-assisted separation of ssDNA

Ningyi Xu,
Xinyue Ma,
Yue Cao,
Huan Wang,
Han Wu,
Hongru Zheng,
Chuanyu Yang,
Ruiqi Zou,
Jiayi Liu,
Chunyan Sun,
Yuan Yuan

Affiliations

Ningyi Xu: Department of Food Quality and Safety, College of Food Science and Engineering, Jilin University, Changchun, 130062, China
Xinyue Ma: Department of Food Quality and Safety, College of Food Science and Engineering, Jilin University, Changchun, 130062, China
Yue Cao: Department of Food Quality and Safety, College of Food Science and Engineering, Jilin University, Changchun, 130062, China
Huan Wang: Department of Food Quality and Safety, College of Food Science and Engineering, Jilin University, Changchun, 130062, China
Han Wu: Jilin Province Product Quality Supervision and Inspection Institute, Changchun, 130103, China
Hongru Zheng: Jilin Province Product Quality Supervision and Inspection Institute, Changchun, 130103, China
Chuanyu Yang: Department of Food Quality and Safety, College of Food Science and Engineering, Jilin University, Changchun, 130062, China
Ruiqi Zou: Department of Food Quality and Safety, College of Food Science and Engineering, Jilin University, Changchun, 130062, China
Jiayi Liu: Department of Food Quality and Safety, College of Food Science and Engineering, Jilin University, Changchun, 130062, China
Chunyan Sun: Department of Food Quality and Safety, College of Food Science and Engineering, Jilin University, Changchun, 130062, China; Corresponding author.
Yuan Yuan: Department of Food Quality and Safety, College of Food Science and Engineering, Jilin University, Changchun, 130062, China; Corresponding author.

Journal volume & issue: Vol. 2, no. 3
pp. 276 – 283

Abstract

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Fluorescent aptasensor was developed for acrylamide (AAm) detection by utilizing the adsorption effect of Au nanoparticles (AuNPs) and fluorescence properties of SYBR Green I (SGI) towards double-stranded DNA (dsDNA). Compared to the binding of aptamer with AAm, the higher affinity of aptamer with cDNA may facilitate a structure switching from the aptamer/AAm complex to aptamer/cDNA dsDNA. The free aptamers were adsorbed onto AuNPs and separated by centrifugation. Subsequently, SGI was introduced as a fluorescent reporter for quantitative detection. Compared to conventional AuNPs-based colorimetric detection, the sensitivity of this strategy was improved by 3.18-fold in the range of 0.005–50 mg/L with a low detection limit of 4.68 μg/L. The method has been successfully applied to analyze fried twists and biscuits. Notably, it is a low-cost and general method that provides guidance for the development of rapid screening technology in the field.

Published in Advanced Agrochem

ISSN: 2773-2371 (Online)
Publisher: KeAi Communications Co., Ltd.
Country of publisher: China
LCC subjects: Agriculture: Agriculture (General); Science: Chemistry: Organic chemistry: Biochemistry
Website: https://www.keaipublishing.com/en/journals/advanced-agrochem/

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