Molecular Genotyping of Giardia duodenalis in Humans in the Yazd County, Central of Iran

Abstract

Background: To investigate Molecular Genotyping of Giardia duodenalis in Humans in the Yazd County, Central of Iran. Methods: Total of 35 fecal samples were collected from patients referred to Yazd central laboratory from February to July 2022. All the samples were included in this study after microscopic observation of G duodenalis. DNA samples were extracted using related kit and were analyzed by Nano Drop. The molecular assessment was carried out using semi-nested PCR using the target gene of gdh. All amplified samples were sequenced using Sanger method. The sequences were analyzed by BLAST for assemblage identification. Results: Out of 35 samples, 24 (68.57%) and 11 (31.43%) were male and female, respectively. All included samples were amplified using the specific gdh primer pair. The molecular analysis showed 17 isolates (48.57%) as assemblage BIV, 8 isolates (22.86%) as assemblage BIII, 6 isolates (17.14%) as assemblage AII and 4 isolates (11.43%) as assemblage AIII (p<0.05). Conclusions: The molecular analysis conducted in this study showed that assemblages A and B are the most prevalent in Central Iran. The molecular identification of G duodenalis isolates from animals and implementing control programs is strongly recommended as a preventive measure to limit the transmission of giardiasis to humans.

Keywords

• Giardia duodenalis
• Genotyping
• Assemblages
• Iran