Veterinarski Glasnik (Jan 2020)

Platelet-poor plasma of athletes is a potent inducer of myogenic differentiation of C2C12 myoblasts

  • Maslovarić Irina S.,
  • Ilić Vesna Lj.,
  • Stančić Ana Z.,
  • Santibanez Juan F.,
  • Trivanović Drenka I.,
  • Drvenica Ivana T.,
  • Krstić Jelena S.,
  • Mojsilović Slavko B.,
  • Okić-Đorđevič Ivana N.,
  • Bugarski Diana S.

DOI
https://doi.org/10.2298/VETGL190414019M
Journal volume & issue
Vol. 74, no. 1
pp. 18 – 33

Abstract

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Introduction. Blood products, i.e. platelet rich plasma (PRP), leukocyte-poor plasma (PRP) and platelet poor plasma (PPP), have previously been used to improve muscle regeneration. In this study, six months’ frozen-stored PPP of individuals who practiced different types of physical exercise was analysed; it could steer mouse C2C12 myoblast cells towards proliferation, migration and myogenic differentiation, and it could affect the morphology/shape of myotubes. Materials and Methods. PPP of male Olympic weightlifters, football players and professional folk dancers, aged 15-19, was collected 12 h post-training and stored for 6 months at -20°C. C2C12 cell proliferation was assessed by MTT test, motility by scratch assay, myogenic differentiation by myotube formation and gelatinase activity by gel-zymography. Results and Conclusions. PPP induced proliferation and migration of C2C12 cells. Proliferative capacity was as follows: weightlifters > dancers > football players; mean migratory capacity was: weightlifters = dancers > football players. PPP induced formation of myotubes; significant inter-individual variations were detected: PPP from weightlifters induced formation of round myotubes, and PPP from football players and dancers induced formation of elongated myotubes. The mean myotube area was as follows: football players > dancers > weightlifters. PPP gelatinolytic activity was observed; it was negatively correlated with C2C12 myoblast proliferation. These results provide general but distinct evidence that PPP of individuals practicing certain types of exercise can specifically modify myoblast morphology/function. This is significant for explaining physiological responses and adaptations to exercise. In conclusion, long-term, frozen-stored PPP preserves its potential to modify myoblast morphology and function. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. 175062]

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