Effect of vitreous cavity transplantation of bone marrow mesenchymal stem cells on optic nerve protection in rat glaucoma model

Abstract

AIM:To investigate the neuroprotective effect of vitreous cavity transplantation of bone marrow mesenchymal stem cells on rat glaucoma model. METHODS: Rat primary bone marrow mesenchymal stem cells were extracted and identified; Lewis rats were randomly divided into 3 groups: Control group, Glaucoma group and bone marrow mesenchymal stem cell group(BMMSCs group), and rat glaucoma model were made: the left eye was the experimental eye(glaucoma), the right eye is the control eye, and identified them. Then vitreous cavity transplantation of bone marrow mesenchymal stem cells were performed; HE staining was used to observe the morphology of the retina; The number of retinal ganglion cells was detected by immunofluorescence; TUNEL staining was used to observe the apoptosis of retinal ganglion cells; The expression of IGF1 and BDNF protein in retinal tissue was detected by Western blot. RESULTS: The intraocular pressure of the experimental eyes of the rats was significantly higher than that of the control eyes(PPPPPPPCONCLUSION:Bone marrow mesenchymal stem cell vitreous cavity transplantation can improve glaucoma in rats and protect retinal ganglion cells.

Keywords

• bone marrow mesenchymal stem cells
• vitreous cavity transplantation
• glaucoma
• optic nerve
• protective effect