Frontiers in Immunology (Jul 2021)

Immunopeptidomics-Guided Warehouse Design for Peptide-Based Immunotherapy in Chronic Lymphocytic Leukemia

  • Annika Nelde,
  • Annika Nelde,
  • Annika Nelde,
  • Yacine Maringer,
  • Yacine Maringer,
  • Yacine Maringer,
  • Tatjana Bilich,
  • Tatjana Bilich,
  • Tatjana Bilich,
  • Helmut R. Salih,
  • Helmut R. Salih,
  • Malte Roerden,
  • Malte Roerden,
  • Malte Roerden,
  • Jonas S. Heitmann,
  • Jonas S. Heitmann,
  • Ana Marcu,
  • Jens Bauer,
  • Jens Bauer,
  • Marian C. Neidert,
  • Claudio Denzlinger,
  • Gerald Illerhaus,
  • Walter Erich Aulitzky,
  • Hans-Georg Rammensee,
  • Hans-Georg Rammensee,
  • Hans-Georg Rammensee,
  • Juliane S. Walz,
  • Juliane S. Walz,
  • Juliane S. Walz,
  • Juliane S. Walz

DOI
https://doi.org/10.3389/fimmu.2021.705974
Journal volume & issue
Vol. 12

Abstract

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Antigen-specific immunotherapies, in particular peptide vaccines, depend on the recognition of naturally presented antigens derived from mutated and unmutated gene products on human leukocyte antigens, and represent a promising low-side-effect concept for cancer treatment. So far, the broad application of peptide vaccines in cancer patients is hampered by challenges of time- and cost-intensive personalized vaccine design, and the lack of neoepitopes from tumor-specific mutations, especially in low-mutational burden malignancies. In this study, we developed an immunopeptidome-guided workflow for the design of tumor-associated off-the-shelf peptide warehouses for broadly applicable personalized therapeutics. Comparative mass spectrometry-based immunopeptidome analyses of primary chronic lymphocytic leukemia (CLL) samples, as representative example of low-mutational burden tumor entities, and a dataset of benign tissue samples enabled the identification of high-frequent non-mutated CLL-associated antigens. These antigens were further shown to be recognized by pre-existing and de novo induced T cells in CLL patients and healthy volunteers, and were evaluated as pre-manufactured warehouse for the construction of personalized multi-peptide vaccines in a first clinical trial for CLL (NCT04688385). This workflow for the design of peptide warehouses is easily transferable to other tumor entities and can provide the foundation for the development of broad personalized T cell-based immunotherapy approaches.

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