Jichu yixue yu linchuang (Sep 2020)

hsa-miR-4483 promotes the proliferation and migration of breast cancer cell lines

  • YANG De-quan, LI Chang-you, CUI Jun-wei, WEI Wei, WANG Shao-ping

Journal volume & issue
Vol. 40, no. 9
pp. 1230 – 1236

Abstract

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Objective To explore the expression and function of hsa-miR-4483 in breast cancer cells. Methods Using TargetScan to find out how can-miR-4483 act on the c-Myc gene, and RT-qPCR verified the expression of hsa-miR-4483. Transfected MCF-7 and MDA-MB-231 and set up NC-negative controls, Western blot was used to detect the expression of the hsa-miR-4483 gene, MTT assay and Transwell chamber experiment were applied to verify the proliferation and migration of breast cancer cells. Results The expression of hsa-miR-4483 was significantly lower (P＜0.001, P＜0.01) in MCF-7 and MDA-MB-231(P＜0.001, P＜0.01) The up-regulation of c-myc in MCF-7 and MDA-MB-231 promoted cell proliferation and migration (P＜0.001). Conclusions hsa-miR-4483 can promote the proliferation and migration of breast cancer cell lines, which plays a regulatory role in the development of breast cancer and its prognosis.

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