Highly Effective xMAP Multiplex Assay for the Detection and Identification of Hemorrhagic Fever Agents, Including Ebola Virus

Abstract

Developed has been the oligonucleotide liquid biochip based on xMAP technology, designed for the laboratory detection of particularly dangerous viral pathogens such as Ebola and Marburg filoviruses, and Machupo , Junin, and Lassa arenaviruses. The suggested approach allows for the detection of up to 100 viral genome equivalents in a sample. The sensitivity and specificity of oligonucleotide biochip is 100 % when the laboratory panels of positive and negative samples are used. These results indicate that the xMAP multiplexing for the detection and identification of tropical hemorrhagic fever agents, including Ebola virus, is not inferior to the conventional method such as real-time RT-PCR and can be applied for evaluation of viral load, and further on can easily be expanded for both the analysis of new viral agents and for the detection of critical mutations in viral genomes.

Keywords

• xmap
• филовирусы
• эбола
• марбург
• аренавирусы
• ласса
• хунин
• мачупо
• пцр
• filoviridae
• ebola
• marburg
• arenaviruses
• lassa
• junin
• machupo
• pcr