Molecular detection of Cryptosporidium parvum in chicken in Al-Diwaniya province

Abstract

This study were conducted in Al-Diwaniya province, in south Iraq during the period from Februaryto July 2019 to determine the rate of infection of Cryptosporidium parvum in domestic chicken, study the effect of some epidemiological factors such as sex and months on the rate of infection, addition to the molecular identification of Cryptococcus parvum by amplification HSP70 gene by conventional PCR. Number of collected fecal sample was 210 from domestic chicken and stained by Ziehl-Neelsen stain. The results of the microscopic examination showed that 108(51.4%) out of 210 fecal samples were infected with Cryptosporidium spp. The statistical analysis founded no marked difference in prevalence of infection between sexes. Significant difference was recorded between infection rate during the months of the study and higher prevalence of infection rate was observed in March 11.9%, while lowest infection rate was observed in July 5.23%. and June 5.23%. Genomic DNA was extracted from 108 fecal samples and HSP70 gene for C. parvum was amplified by PCR. PCR technique is showed that out of 108 fecal samples 21.3% were positive for Cryptosporidium parvum.

Keywords

• cryptosporidium parvum
• domestic chicken
• pcr
• al-diwaniya